PMID: 41245Sep 1, 1979

Requirement for membrane potential in injection of phage T4 DNA

Proceedings of the National Academy of Sciences of the United States of America
B Labedan, E B Goldberg

Abstract

The first stages of infection by phage T4 may be divided into energy-dependent and energy-independent processes. Irreversible adsorption, unplugging, and initial exposure of the DNA terminus may occur at 4 degrees C, or at 37 degrees C in bacteria whose energy-yielding metabolism has been poisoned. DNA injection into the cytoplasm needs higher temperatures and energy from the host cell. The nature of this energy requirements was deduced from the use of metabolic inhibitors. Our results show that T4 DNA injection specifically requires the presence of a protonmotive force across the cytoplasmic membrane of the host. Moreover, the chemical gradient (delta pH) does not appear to be essential, but the membrane potential (delta psi) is required.

References

Feb 1, 1984·Journal of Bioenergetics and Biomembranes·B Labedan, Lucienne Letellier
May 1, 1981·Biulleten' eksperimental'noĭ biologii i meditsiny·M I Bukrinskiĭ, A G Sabel'nikov
Jan 1, 1987·The Journal of Membrane Biology·E Padan, S Schuldiner
Jan 4, 1994·Biochimica Et Biophysica Acta·R PalmenK J Hellingwerf
Jan 1, 1994·Progress in Biophysics and Molecular Biology·A G Sabelnikov
Jan 1, 1983·Pharmacology & Therapeutics·L Carrasco, J C Lacal
Jan 1, 1989·Pharmacology & Therapeutics·L CarrascoJ L Castrillo
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B Labedan, Lucienne Letellier
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M E Bayer, M H Bayer
Apr 1, 1992·Journal of Biomolecular Structure & Dynamics·I S Gabashvili, Grosberg AYu
Jan 27, 2009·Applied and Environmental Microbiology·Lejla ImamovicMaite Muniesa
Dec 1, 2000·Applied and Environmental Microbiology·D R KadavyT A Kokjohn
Jul 8, 2009·Journal of Bacteriology·Maria João FriasMário Ramirez
Nov 4, 2008·Journal of Bacteriology·Gerardo L PerezStanley Maloy
Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·Fumio ArisakaMichael G Rossmann
Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bo HuJun Liu
Dec 1, 1989·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·A I Vinnikov, V S Zueva
Aug 24, 2007·Current Opinion in Microbiology·Paul Grayson, Ian J Molineux
Dec 3, 2015·BMC Microbiology·Mariángeles Briggiler MarcóAndrea Quiberoni
Aug 13, 2004·Molecular Microbiology·Priscilla KempIan J Molineux
Mar 28, 1980·Biochemical and Biophysical Research Communications·B LabedanE B Goldberg
Mar 7, 2006·Gene·Víctor González-HuiciJosé M Hermoso
Apr 30, 1981·Biochemical and Biophysical Research Communications·E Santos, H R Kaback
Dec 27, 2011·International Journal of Food Microbiology·Claudia PicozziRoberto Foschino
Apr 18, 2018·Journal of Bacteriology·Tamara Reyes-RoblesAndrew Camilli
Sep 2, 1991·FEBS Letters·Y S TarahovskyY V Kim
Jan 1, 1980·Journal of Supramolecular Structure·T H Wilson, E C Lin
Feb 7, 2013·Nature Reviews. Microbiology·Ian J Molineux, Debabrata Panja
Jun 30, 2017·Frontiers in Microbiology·Kai ChengYi Jun Zhao
Aug 2, 2014·Molecular BioSystems·Nusrat J M SanghamitraTakafumi Ueno
Jan 1, 1983·The Journal of Membrane Biology·H R Kaback
Oct 23, 2019·Journal of Bacteriology·Poutoum SamireLaetitia Houot

Citations

Feb 1, 1976·Journal of Bioenergetics·M ToroS Estrada-O
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M J Weiss, S E Luria
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R W Hendrix
Nov 1, 1977·Journal of Molecular Biology·D B Oliver, E B Goldberg
Jul 15, 1975·FEBS Letters·B Leipold, P H Hofschneider
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Hirokawa
Dec 29, 1971·Nature: New Biology·J OrtĭnC Vasquez
Jan 1, 1969·Journal of Theoretical Biology·V Zárybnický
May 1, 1952·The Journal of General Physiology·A D HERSHEY, M CHASE
Jul 1, 1955·Archives of Biochemistry and Biophysics·J R CHRISTENSEN, L J TOLMACH

Related Concepts

Alkalescens-Dispar Group
Uncoupling Agents
Valinomycin
Resting Potentials
DNA, Viral
Virus Replication
Ions
Colicin E8
Hydrogen-Ion Concentration
T-Phages

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.