Requirement for protein synthesis during embryonic genome activation in mice

Molecular Reproduction and Development
Q Wang, K E Latham

Abstract

Embryonic genome activation (EGA) occurs by the 2-cell stage in mouse embryos. To understand the molecular basis of EGA, it is important to determine whether EGA can be supported by maternally inherited factors or if it requires the synthesis of additional transcription factors. We used a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method to test whether protein synthesis is required for the transcriptional activation of six housekeeping genes (U2afbp-rs, Hprt, Pdha1, Prps1, Odc, and Cox7c). Cycloheximide treatment reduced the expression of these mRNAs in 2-cell embryos to the same degree as alpha-amanitin treatment. Cycloheximide treatment did not reduce the expression of maternally inherited mRNAs, indicating that its effect is specific for transcription-dependent gene expression. These results contrast with earlier results reported for the Hsp70 gene. This difference may reflect differences in promoter requirements. We conclude that protein synthesis is required for the activation of most, if not all, housekeeping genes in the mouse embryo, and that the time of EGA may be controlled, in part, by the regulated recruitment of maternal mRNAs encoding key transcription factors.

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Citations

Mar 21, 1998·Developmental Genetics·H J ClarkeO A Mohamed
Feb 9, 2002·Molecular Reproduction and Development·Jean W WilliamsKeith E Latham
Jan 29, 2013·Molecular Aspects of Medicine·Lei LiJurrien Dean
Aug 3, 2004·Biology of Reproduction·Shun-Ichiro KageyamaFugaku Aoki
Sep 11, 2007·The Journal of Reproduction and Development·Naojiro MinamiSatoshi Tsukamoto
Mar 25, 2008·The Journal of Reproduction and Development·Toshiki MatsuokaKazuya Matsumoto
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Jan 6, 2007·Human Cell·Toshio HamataniYasunori Yoshimura
Apr 4, 2008·Molecular Reproduction and Development·Andrej SusorMichal Kubelka
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Dec 11, 2014·Fertility and Sterility·Catherine A VandeVoortKeith E Latham
Oct 28, 2003·Molecular Reproduction and Development·Sezen AratSteven L Stice
Jan 28, 2003·Molecular Reproduction and Development·Fugaku AokiRichard M Schultz
Sep 13, 2005·Molecular Reproduction and Development·Rita VassenaKeith E Latham
Apr 25, 2014·Journal of Assisted Reproduction and Genetics·Gibence Rose Winnie HendersonShivaji Sisinthy
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Mar 27, 2003·Molecular Reproduction and Development·Anthony D MetcalfeDaniel R Brison
Sep 3, 2005·Developmental Biology·Kentaro T HaraFugaku Aoki
Jan 24, 2002·Genetics·Vanessa E GurtuSean M Baker

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