Requirement for Rho-mediated myosin light chain phosphorylation in thrombin-stimulated cell rounding and its dissociation from mitogenesis.

The Journal of Biological Chemistry
M MajumdarJ H Brown

Abstract

Thrombin treatment causes a dose-dependent rounding of 1321N1 astrocytoma cells. This cytoskeletal response is rapid, peaking 2 h after thrombin stimulation, and reverses by 50% after 24 h. The thrombin receptor peptide SFLLRNP also induces cell rounding, whereas other G protein-linked receptor agonists such as carbachol, lysophosphatidic acid, or bradykinin fail to do so. Results of studies using pharmacological inhibitors do not support a requirement for phosphatidylinositol 3-kinase, mitogen-activated protein kinase, or Ca2+ mobilization in this response. Inhibition of protein kinase C or tyrosine kinase produces minimal blockade. Pertussis toxin treatment is also without effect. However, thrombin-induced rounding is fully blocked by the C3 toxin from Clostridium botulinum, which specifically ADP-ribosylates and inactivates the small G protein Rho. Thrombin also leads to a rapid, 2.4-fold increase in 32P incorporation into myosin light chain while carbachol does not. Myosin phosphorylation, like cell rounding is inhibited by inactivation of Rho with C3 exoenzyme, suggesting that myosin phosphorylation is necessary for this cytoskeletal response. This is supported by the observation that thrombin-induced rounding is also bloc...Continue Reading

References

Apr 1, 1992·The Journal of Clinical Investigation·D T HungS R Coughlin
May 1, 1992·Molecular Biology of the Cell·A Hall
Jul 1, 1991·The American Journal of Physiology·R B Wysolmerski, D Lagunoff
Jul 1, 1991·The Journal of Cell Biology·A K WilsonP de Lanerolle
Dec 15, 1988·Biochemical and Biophysical Research Communications·J NishimuraC van Breemen
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K M TrybusS Lowey
Oct 20, 1995·The Journal of Biological Chemistry·A M BuhlG L Johnson
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Aug 25, 1995·The Journal of Biological Chemistry·A M AragayM I Simon
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Jan 1, 1993·The Biochemical Journal·V Vouret-CraviariJ Pouysségur
Feb 15, 1993·Biochemical and Biophysical Research Communications·C F ReillyE J Mayer
Apr 1, 1993·The Journal of Cell Biology·V J LaMorteJ R Feramisco
Jan 15, 1996·The Biochemical Journal·R J GrandP W Grabham
May 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G R Post, J H Brown
Jul 19, 1996·The Journal of Biological Chemistry·L R CollinsJ H Brown
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Nov 11, 1996·The American Journal of Physiology·G HechtP D Lanerolle
Apr 18, 1997·The Journal of Biological Chemistry·M C GongA P Somlyo
Apr 21, 1997·The Journal of Cell Biology·R L KlemkeD A Cheresh

❮ Previous
Next ❯

Citations

Feb 14, 2006·IEE Proceedings. Nanobiotechnology·P D Chantler, S R Wylie
Mar 30, 2001·The Journal of Biological Chemistry·S A SagiJ H Brown
Jun 16, 2011·Stem Cells and Development·Wenjing WangJo-Anna Reems
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
May 6, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Leshuai W Zhang, Nancy A Monteiro-Riviere
Feb 18, 2005·Acta Pharmacologica Sinica·Jun Ren, Cindy X Fang
Oct 3, 2008·Molecular Interventions·Colin T WalshJoan Heller Brown
Feb 26, 2000·Infection and Immunity·S Zhang, C W Maddox
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·V P SahJ H Brown
May 23, 2008·American Journal of Physiology. Cell Physiology·Andrew E BlumGeorge R Dubyak
Mar 2, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hisashi ShirakawaShuji Kaneko
Apr 5, 2003·Biological Chemistry·Hong Wang, Georg Reiser
May 16, 2001·Biochemical and Biophysical Research Communications·M ZiegerR Kaufmann
Aug 9, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Colin T WalshJoan Heller Brown
Aug 20, 2003·The Journal of Investigative Dermatology·Glynis ScottRujiing Han
Jan 22, 2008·British Journal of Pharmacology·S M KredaE R Lazarowski
Feb 1, 2005·The Journal of Surgical Research·Daphne P Ly, Siobhan A Corbett
Sep 15, 2006·Regulatory Toxicology and Pharmacology : RTP·Jane K HeffernanMark C Rogge
Dec 29, 2010·Journal of Cardiovascular Disease Research·Guang YangRudolf Lucas
Dec 10, 1999·Molecular and Cellular Neurosciences·J FritscheC E Bandtlow
Feb 9, 2000·Genomics·L NeilsonD P Green
Nov 30, 2010·Experimental Cell Research·Anna A BirukovaKonstantin G Birukov
Mar 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·María L Vitale, M Eloísa Carbajal
May 15, 2004·The Journal of Biological Chemistry·Qin WangRichard R Neubig
Oct 12, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Richard T ClementsPeter A Vincent
May 12, 2007·Journal of Cellular Biochemistry·Piet W L TasNorbert Roewer
Dec 8, 2007·Journal of Cellular Physiology·Anna A BirukovaKonstantin G Birukov
May 10, 2005·The Journal of Biological Chemistry·Joseph N McLaughlinHeidi E Hamm
Jun 27, 2001·International Journal of Impotence Research·K ChitaleyT M Mills
Apr 13, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna A BirukovaKonstantin G Birukov
Apr 10, 2003·The Journal of Biological Chemistry·Sheldon M JosephGeorge R Dubyak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.