Requirement for the budding yeast polo kinase Cdc5 in proper microtubule growth and dynamics

Eukaryotic Cell
Chong J ParkKyung S Lee

Abstract

In many organisms, polo kinases appear to play multiple roles during M-phase progression. To provide new insights into the function of the budding yeast polo kinase Cdc5, we generated novel temperature-sensitive cdc5 mutants by mutagenizing the C-terminal noncatalytic polo box domain, a region that is critical for proper subcellular localization. One of these mutants, cdc5-11, exhibited a temperature-sensitive growth defect with an abnormal spindle morphology. Strikingly, provision of a moderate level of benomyl, a microtubule-depolymerizing drug, permitted cdc5-11 cells to grow significantly better than the isogenic CDC5 wild type in a FEAR (cdc Fourteen Early Anaphase Release)-independent manner. In addition, cdc5-11 required MAD2 for both cell growth and the benomyl-remedial phenotype. These results suggest that cdc5-11 is defective in proper spindle function. Consistent with this view, cdc5-11 exhibited abnormal spindle morphology, shorter spindle length, and delayed microtubule regrowth at the nonpermissive temperature. Overexpression of CDC5 moderately rescued the spc98-2 growth defect. Interestingly, both Cdc28 and Cdc5 were required for the proper modification of the spindle pole body components Nud1, Slk19, and Stu2 in...Continue Reading

References

Dec 1, 1991·Genes & Development·S LlamazaresC E Sunkel
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Mar 1, 1996·The Journal of Cell Biology·J A WaddleJ A Cooper
Apr 16, 1998·Current Biology : CB·A C BishopK M Shokat
Jun 25, 1998·Molecular and Cellular Biology·Y W QianJ L Maller
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·K S LeeR L Erikson
Nov 20, 1998·Molecular and Cellular Biology·L ChengC F Hardy
Jul 31, 1999·The Journal of Cell Biology·X ZengW S Saunders
Apr 4, 2001·The Journal of Cell Biology·P P BuddeR Heald
Apr 13, 2001·Current Biology : CB·J W HudsonJ W Dennis
Apr 20, 2001·The Journal of Cell Biology·F SeverinT Hyman
Jun 14, 2002·Trends in Cell Biology·Jordan W Raff
Jun 21, 2002·Trends in Cell Biology·Kazuhisa KinoshitaAnthony A Hyman
Aug 9, 2002·Molecular and Cellular Biology·Frederic R Yarm
Jul 11, 2003·Developmental Cell·Martina CasenghiErich A Nigg
Nov 8, 2003·Science·Gislene Pereira, Elmar Schiebel
Jun 3, 2004·Nature Reviews. Molecular Cell Biology·Francis A BarrErich A Nigg
Oct 29, 2004·Molecular and Cellular Biology·Jung-Eun ParkKyung S Lee
Jan 11, 2005·Oncogene·Drew M LoweryMichael B Yaffe
May 28, 2005·The EMBO Journal·Satoshi AsanoKyung S Lee
Apr 22, 2006·Cell Cycle·Barbara C M van de Weerdt, René H Medema

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Citations

Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hery RatsimaDamien D'Amours
May 15, 2010·Cell Division·Jorrit M Enserink, Richard D Kolodner
Dec 15, 2010·Cell Division·Ayse K CaydasiGislene Pereira
Dec 30, 2011·Cell Division·Kobi J Simpson-Lavy, Michael Brandeis
Apr 12, 2012·Genetics·Mark Winey, Kerry Bloom
May 27, 2016·Molecular Biology of the Cell·Prashant K MishraMunira A Basrai
May 8, 2018·Cytoskeleton·Matt GreenleeRita K Miller
Aug 4, 2018·Journal of Cell Science·Erica RaspelliRoberta Fraschini
Aug 5, 2017·Current Genetics·Vladimir V Botchkarev, James E Haber
Nov 23, 2020·Current Genetics·Cinzia KlemmGuðjón Ólafsson

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