PMID: 4370340Aug 1, 1974

Requirement of 3'-terminal poly(adenylic acid) for the infectivity of poliovirus RNA

Proceedings of the National Academy of Sciences of the United States of America
D H Spector, D Baltimore

Abstract

Ribonuclease H (EC 3.1.4.34) has been used to remove selectively much of the 3'-terminal poly(adenylic acid) [poly(A)] from poliovirus RNA by treating the RNA with the enzyme in the presence of poly(dT). Over 80% of the poly(A) could be removed and the residuum was found as oligo(A) stretches attached to many or all of the viral RNA molecules. Reduction of the size of the poly(A) markedly decreased the specific infectivity of poliovirus RNA, indicating that poly(A) is necessary to the infectivity of the RNA. The virions in plaques deriving from infection with treated RNA have a normal amount and size of poly(A), indicating that mechanisms exist in infected cells to regenerate normal length poly(A) from truncated poly(A).

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Related Concepts

Alkalescens-Dispar Group
SDS-PAGE
Virus Replication
Polynucleotides
Bacteriophage Plaque Assay
RNASEH1
Human poliovirus 3
Alkaline Ribonuclease
Tritium
Cell Division Phases

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