PMID: 4370340Aug 1, 1974

Requirement of 3'-terminal poly(adenylic acid) for the infectivity of poliovirus RNA

Proceedings of the National Academy of Sciences of the United States of America
D H Spector, D Baltimore


Ribonuclease H (EC has been used to remove selectively much of the 3'-terminal poly(adenylic acid) [poly(A)] from poliovirus RNA by treating the RNA with the enzyme in the presence of poly(dT). Over 80% of the poly(A) could be removed and the residuum was found as oligo(A) stretches attached to many or all of the viral RNA molecules. Reduction of the size of the poly(A) markedly decreased the specific infectivity of poliovirus RNA, indicating that poly(A) is necessary to the infectivity of the RNA. The virions in plaques deriving from infection with treated RNA have a normal amount and size of poly(A), indicating that mechanisms exist in infected cells to regenerate normal length poly(A) from truncated poly(A).


Jan 1, 1990·Archives of Virology·L A Seal, R M Jamison
Jan 1, 1980·Archives of Virology·S Katow, T Matsuno
Sep 9, 1977·Molecular and Cellular Biochemistry·T P KarpetskyC C Levy
Jan 1, 1979·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·S Kit
Feb 1, 1991·Brain Research. Molecular Brain Research·E D LampertiL Villa-Komaroff
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·James B FlaneganD Baltimore
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·James B Flanegan, D Baltimore
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N C Andrews, D Baltimore
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J TartagliaD L Nuss
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V Volloch
Oct 1, 1976·Nucleic Acids Research·D FrisbyA Porter
Mar 25, 1980·Nucleic Acids Research·G R LarsenEckard Wimmer
Jan 1, 1979·Microbiology and Immunology·H ShibutaY Yogo
Nov 20, 2012·Molekuliarnaia biologiia·P M ChumakovN V Tikunova
Jul 12, 2007·Virology Journal·Nidia H De Jesus
Jan 1, 1980·Pharmacology & Therapeutics·L Carrasco, A E Smith
Aug 1, 1975·Life Sciences·C C Levy
Dec 6, 2011·Current Opinion in Virology·Sushma A Ogram, James B Flanegan
Dec 14, 1978·Biochemical and Biophysical Research Communications·S I Koliais, N J Dimmock
Nov 19, 2003·Biochemical and Biophysical Research Communications·Ana Lorena Gutiérrez-EscolanoJavier Hernández-Acosta
Dec 20, 1976·Biochemical and Biophysical Research Communications·E A NeuweltC C Levy
Apr 28, 1978·Biochemical and Biophysical Research Communications·D E Hruby
May 26, 2004·Microbes and Infection·Kristin M Bedard, Bert L Semler
May 23, 2001·The Journal of General Virology·C Polacek, A M Lindberg
Dec 24, 2013·Journal of Virological Methods·Natallia V LazouskayaPeter A Barton
Jan 17, 2015·Virus Research·Aniko V Paul, Eckard Wimmer


Aug 12, 1966·Biochemical and Biophysical Research Communications·G KochJ M Bishop
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·Y Yogo, Eckard Wimmer
Apr 11, 1973·Nature: New Biology·Y Yogo, Eckard Wimmer
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S PerlmanS Penman
Jan 1, 1973·Annual Review of Biochemistry·R A Weinberg
Jun 28, 1967·Journal of Molecular Biology·D H BishopS Spiegelman

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