PMID: 8605318Feb 1, 1996Paper

Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus

Plant Molecular Biology
C JiangJ Singh

Abstract

Mutation of the core pentamer, CCGAC, of two putative low temperature responsive elements (LTREs) in the 5'-proximal region for the winter Brassica napus cold-induced gene BN115 was carried out. Analyses of transient expression of the resultant mutated BN115 promoter-GUS fusions revealed the loss of low-temperature regulation by the promoter. This indicates that the CCGAC sequence is critical to the low-temperature response in the BN115 gene. In contrast, mutation of two G-boxes, CACGTG, staggered between the LTREs in the same region of the promoter did not alter cold-inducible gene expression. Replacement of a possible enhancer region of the BN115 promoter with the enhancer from the CaMV 35S promoter resulted in a several-fold increase in low temperature-induced GUS activity.

References

Dec 1, 1993·Plant Physiology·D P HorvathM F Thomashow

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Citations

Sep 18, 2008·Plant Cell Reports·Junling HuaiGuoying Wang
Oct 10, 2006·Biotechnology Letters·Rubén AlcázarTeresa Altabella
Jan 24, 2007·Plant Molecular Biology·Tatiana SuprunovaAbraham Korol
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hou-Sung JungJoanne Chory
Jul 31, 2013·Omics : a Journal of Integrative Biology·Manali MishraGirdhar K Pandey
Dec 21, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Kyonoshin MaruyamaKazuko Yamaguchi-Shinozaki
May 11, 2004·Journal of Experimental Botany·An MichielsMark Tucker
Jul 9, 2011·Journal of Experimental Botany·Charu Lata, Manoj Prasad
Jul 19, 2008·Plant & Cell Physiology·Mohamed BadawiMario Houde
Aug 15, 2002·Plant Physiology·Dong-Woog ChoiTimothy J Close
Jan 8, 2009·Plant Physiology·Kazuo NakashimaKazuko Yamaguchi-Shinozaki
Jul 18, 2001·Plant Physiology·X M YuS B Wiseman
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Michael F. Thomashow
May 4, 2006·Annual Review of Plant Biology·Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
Dec 22, 2010·BMC Genomics·Francesco LicausiPierdomenico Perata
Jan 18, 2013·BMC Genomics·Cuifang DuanPascal Montoro
May 29, 2008·Genes & Genetic Systems·Naruhito OhnishiKazuhiko Noda

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