Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis.

The Journal of Biological Chemistry
Yongqing LiJian Hua

Abstract

Copines are highly conserved proteins with lipid-binding activities found in animals, plants, and protists. They contain two calcium-dependent phospholipid binding C2 domains at the amino terminus and a VWA domain at the carboxyl terminus. The biological roles of most copines are not understood and the biochemical properties required for their functions are largely unknown. The Arabidopsis copine gene BON1/CPN1 is a negative regulator of cell death and defense responses. Here we probed the potential biochemical activities of BON1 through mutagenic studies. We found that mutations of aspartates in the C2 domains did not alter plasma membrane localization but compromised BON1 activity. Mutation at putative myristoylation residue glycine 2 altered plasma membrane localization of BON1 and rendered BON1 inactive. Mass spectrometry analysis of BON1 further suggests that the N-peptide of BON1 is modified. Furthermore, mutations that affect the interaction between BON1 and its functional partner BAP1 abolished BON1 function. This analysis reveals an unanticipated regulation of copine protein localization and function by calcium and lipid modification and suggests an important role in protein-protein interaction for the VWA domain of co...Continue Reading

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Citations

Oct 28, 2015·Molecular Plant-microbe Interactions : MPMI·Eva-Maria DischStefan Hoth
Mar 15, 2012·Plant Signaling & Behavior·Mingyue Gou, Jian Hua
Feb 18, 2015·Frontiers in Plant Science·Quentin BruggemanMarianne Delarue
Aug 4, 2016·Genome Génome / Conseil National De Recherches Canada·Baohong ZouJian Hua
May 6, 2020·The Plant Journal : for Cell and Molecular Biology·Qianchao WangYunkun Wu
Oct 10, 2020·Current Biology : CB·Kong ChenYang Zhao

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