Requirement of CHROMOMETHYLASE3 for somatic inheritance of the spontaneous tomato epimutation Colourless non-ripening

Scientific Reports
Weiwei ChenYiguo Hong

Abstract

Naturally-occurring epimutants are rare and have mainly been described in plants. However how these mutants maintain their epigenetic marks and how they are inherited remain unknown. Here we report that CHROMOMETHYLASE3 (SlCMT3) and other methyltransferases are required for maintenance of a spontaneous epimutation and its cognate Colourless non-ripening (Cnr) phenotype in tomato. We screened a series of DNA methylation-related genes that could rescue the hypermethylated Cnr mutant. Silencing of the developmentally-regulated SlCMT3 gene results in increased expression of LeSPL-CNR, the gene encodes the SBP-box transcription factor residing at the Cnr locus and triggers Cnr fruits to ripen normally. Expression of other key ripening-genes was also up-regulated. Targeted and whole-genome bisulfite sequencing showed that the induced ripening of Cnr fruits is associated with reduction of methylation at CHG sites in a 286-bp region of the LeSPL-CNR promoter, and a decrease of DNA methylation in differentially-methylated regions associated with the LeMADS-RIN binding sites. Our results indicate that there is likely a concerted effect of different methyltransferases at the Cnr locus and the plant-specific SlCMT3 is essential for sustain...Continue Reading

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Citations

Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ruie LiuPhilippe Gallusci
Jul 6, 2016·Frontiers in Plant Science·Philippe GallusciGraham B Seymour
Dec 21, 2016·PLoS Genetics·Quentin Gouil, David C Baulcombe
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Nov 13, 2018·The Plant Journal : for Cell and Molecular Biology·Christophe RothanMathilde Causse
Feb 16, 2020·Journal of Experimental Botany·Chen YuanYiguo Hong
Dec 19, 2019·Functional & Integrative Genomics·Xian ZhangYiguo Hong
May 23, 2018·Nature Reviews. Molecular Cell Biology·Huiming ZhangJian-Kang Zhu
Mar 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Todd BlevinsCraig S Pikaard
Feb 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yinwen ZhangRobert J Schmitz
Nov 17, 2020·Frontiers in Plant Science·Seanna Hewitt, Amit Dhingra
Oct 23, 2020·Functional & Integrative Genomics·Gaurav AgarwalRajeev K Varshney
May 11, 2021·Current Opinion in Plant Biology·Javier Brumos
Jun 2, 2021·Biochemical Society Transactions·Wen-Feng Nie
Sep 17, 2021·Nature Reviews. Genetics·James P B Lloyd, Ryan Lister

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Methods Mentioned

BETA
methylation profiling
WGBS
PCR

Software Mentioned

SlCMT3

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