Requirement of domain-domain interaction for conformational change and functional ATP hydrolysis in myosin.

The Journal of Biological Chemistry
Kohji ItoKeiichi Yamamoto

Abstract

Coordination between the nucleotide-binding site and the converter domain of myosin is essential for its ATP-dependent motor activities. To unveil the communication pathway between these two sites, we investigated contact between side chains of Phe-482 in the relay helix and Gly-680 in the SH1-SH2 helix. F482A myosin, in which Phe-482 was changed to alanine with a smaller side chain, was not functional in vivo. In vitro, F482A myosin did not move actin filaments and the Mg2+-ATPase activity of F482A myosin was hardly activated by actin. Phosphate burst and tryptophan fluorescence analyses, as well as fluorescence resonance energy transfer measurements to estimate the movements of the lever arm domain, indicated that the transition from the open state to the closed state, which precedes ATP hydrolysis, is very slow. In contrast, F482A/G680F doubly mutated myosin was functional in vivo and in vitro. The fact that a larger side chain at the 680th position suppresses the defects of F482A myosin suggests that the defects are caused by insufficient contact between side chains of Ala-482 and Gly-680. Thus, the contact between these two side chains appears to play an important role in the coordinated conformational changes and subseque...Continue Reading

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Citations

Sep 17, 2009·Analytical and Bioanalytical Chemistry·Zhiyan SongOlarongbe Olubajo
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kohji ItoKeiichi Yamamoto
Feb 20, 2007·PLoS Computational Biology·Haibo YuQiang Cui
Jan 10, 2012·Journal of Molecular Biology·William A KronertSanford I Bernstein
Jul 16, 2008·Journal of Molecular Biology·Yang YangQiang Cui
Aug 16, 2005·Biophysical Journal·Adam W Van Wynsberghe, Qiang Cui
Mar 29, 2014·Proteins·Onur VarolAlkan Kabakçıoğlu
Oct 12, 2013·Protein Science : a Publication of the Protein Society·Yaroslav V TkachevYuri E Nesmelov
Apr 25, 2006·Biophysical Journal·Semen O YesylevskyyAlexander P Demchenko
Sep 29, 2007·Journal of Molecular Biology·Susan TangJeanette P Schmidt
Jun 9, 2016·Plant & Cell Physiology·Takeshi HaraguchiKohji Ito
Jul 15, 2016·Biophysical Journal·Jinghua GeYuri E Nesmelov
Mar 4, 2017·Journal of the American Chemical Society·Jacek CzubHelmut Grubmüller
Aug 12, 2006·The Journal of Biological Chemistry·Sosuke IwaiShigeru Chaen
May 10, 2007·The Journal of Biological Chemistry·Kohji ItoKeiichi Yamamoto
Mar 19, 2014·The Journal of Biological Chemistry·Takeshi HaraguchiKohji Ito
Nov 7, 2018·Plant & Cell Physiology·Takeshi HaraguchiMotoki Tominaga

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