PMID: 7033109Nov 1, 1981Paper

Requirement of hydrocortisone and insulin for extended proliferation and passage of rat keratinocytes

In Vitro
F L VaughanJ A Uzman

Abstract

A procedure for the preparation and cultivation of rat epidermal basal cells from full thickness skin resulted in greater than 99% viability and 90% plating efficiency. However, attempts to subculture monolayers of these epithelial cells grown in medium with serum as the only supplement were totally unsuccessful. When hydrocortisone and insulin were added to the medium, subcultivation of primary growth was obtained. It was demonstrated that hydrocortisone at concentrations as low as 0.1 micrograms/ml was necessary for at least the initial attachment of the cells to the substrate - an essential step in subcultivation. Increasing concentrations of insulin (0.1 to 50 micrograms/ml) caused the rate of proliferation and the cell density to increase, but insulin alone did not support subcultivation.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M EisingerE de Harven
Jan 1, 1978·In Vitro·I HayashiG Sato
May 1, 1976·In Vitro·A E FreemanK L Kleinfeld
Jun 1, 1976·The Journal of Investigative Dermatology·F L VaughanI A Bernstein
Jun 1, 1971·The Journal of Investigative Dermatology·F L Vaughan, I A Bernstein
Mar 1, 1971·The Journal of Investigative Dermatology·M A Karasek, M E Charlton
Feb 1, 1980·In Vitro·F M PriceK K Sanford
Oct 1, 1955·The Journal of Pathology and Bacteriology·G SZABO
Oct 1, 1957·Experimental Cell Research·H J PHILLIPS, J E TERRYBERRY
May 1, 1952·The Journal of Investigative Dermatology·E J VAN SCOTT

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Citations

Dec 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L I BernstamI A Bernstein
Mar 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·F L VaughanI A Bernstein
Jun 1, 1994·In Vitro Cellular & Developmental Biology. Animal·B HirelA Guillouzo
Jan 1, 1985·Experimental Lung Research·C C LeslieR J Mason
Nov 30, 1992·Biochemical and Biophysical Research Communications·J Xiang, I A Bernstein
Sep 30, 1988·Toxicology and Applied Pharmacology·W W Ku, I A Bernstein
Feb 1, 1988·The Journal of Dermatology·P Verrando, J P Ortonne
Jan 1, 1985·Differentiation; Research in Biological Diversity·R BrownI A Bernstein
Mar 1, 1989·Journal of Cellular Physiology·M Ishihara, H E Conrad
Jun 1, 1987·The Journal of Investigative Dermatology·R BrownI A Bernstein

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