PMID: 4522791Mar 1, 1974

Requirement of initiation factor 3 in the initiation of polypeptide synthesis with N-acetylphenylalanyl-tRNA

Proceedings of the National Academy of Sciences of the United States of America
S D BernalT Nakamoto

Abstract

Initiation factor 3 is required, along with initiation factors 1 and 2, for the incorporation of N-acetylphenylalanine into polypeptides and the formation of N-acetylphenylalanylpuromycin. Initiation factor 3 also strongly stimulates the binding of N-acetylphenylalanyl transfer RNA to isolated 30S ribosomal subunits. Phosphocellulose fractions of initiation factor 3 were found to catalyze N-acetylphenylalanine incorporation differentially with different synthetic messenger RNAs not containing any codons for N-formylmethionine. The results suggest that ribosomes recognize the initiator codon only through the initiator transfer RNA.

References

Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·J S Dubnoff, U Maitra
Aug 23, 1972·Nature: New Biology·M Noll, H Noll
Jul 28, 1972·Journal of Molecular Biology·M Yoshida, P S Rudland
Mar 17, 1973·Biochemical and Biophysical Research Communications·D P SuttleJ M Ravel
Jan 1, 1973·Annual Review of Biochemistry·R Haselkorn, L B Rothman-Denes
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·A E Economou, T Nakamoto
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·T Nakamoto, E Hamel
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·E B Klem, T Nakamoto
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·W T Hsu, S B Weiss
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·M RevelH Greenshpan
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·A J WahbaC Vasquez
Jan 1, 1971·Annual Review of Biochemistry·J Lucas-Lenard
Dec 22, 1971·Nature: New Biology·S Lee-Huang, S Ochoa
Mar 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·T Nakamoto, D Kalokofsky
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Dec 6, 1969·Nature·J Hindley, D H Staples
Oct 1, 1963·Journal of Molecular Biology·M TAKANAMI, T OKAMOTO

Citations

May 1, 1980·Die Naturwissenschaften·K H Nierhaus, H G Wittmann
Feb 16, 1978·Biochimica Et Biophysica Acta·T Nakamoto, B Vogl
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C L Pon, C Gualerzi
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C S Chen, T Nakamoto
Oct 8, 1974·Biochemical and Biophysical Research Communications·S D BernalT Nakamoto
Mar 1, 1980·Archives of Biochemistry and Biophysics·E WickstromE E Williams

Related Concepts

Carbon Radioisotopes
Pedameth
Peptide Chain Initiation, Translational
Peptide Initiation Factors
Endorphenyl
Puromycin Hydrochloride
Ribosomes
Poly(A) Tail
Transfer RNA

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