Requirement of N-myristoyltransferase 1 in the development of monocytic lineage

The Journal of Immunology : Official Journal of the American Association of Immunologists
Anuraag ShrivastavRajendra K Sharma

Abstract

N-myristoyltransferase (NMT) exists in two isoforms, NMT1 and NMT2, that catalyze myristoylation of various proteins crucial in signal transduction, cellular transformation, and oncogenesis. We have recently demonstrated that NMT1 is essential for the early development of mouse embryo. In this report, we have demonstrated that an invariant consequence of NMT1 knock out is defective myelopoesis. Suppressed macrophage colony forming units were observed in M-CSF-stimulated bone marrow cells from heterozygous (+/-) Nmt1-deficient mice. Homozygous (-/-) Nmt1-deficient mouse embryonic stem cells resulted in drastic reduction of macrophages when stimulated to differentiate by M-CSF. Furthermore, to understand the requirement of NMT1 in the monocytic differentiation we investigated the role of NMT, pp60c-Src (NMT substrate) and heat shock cognate protein 70 (inhibitor of NMT), during PMA-induced differentiation of U937 cells. Src kinase activity and protein expression increased during the differentiation process along with regulation of NMT activity by hsc70. NMT1 knock down in PMA treated U937 cells showed defective monocytic differentiation. We report in this study novel observation that regulated total NMT activity and NMT1 is essen...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1992·Developmental Biology·F D Lee
Dec 1, 1991·Analytical Biochemistry·M J King, R K Sharma
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C E GeeT M Roberts
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J B BolenN Rosen
Nov 24, 1983·Nature·I G Haas, M Wabl
Sep 29, 1995·The Journal of Biological Chemistry·C J Glover, R L Felsted
Nov 1, 1995·Journal of the National Cancer Institute·B A MagnusonR K Sharma
Aug 1, 1995·The Journal of Surgical Research·T E GardnerJ M Daly
Nov 1, 1995·Trends in Biochemical Sciences·P J ThomasP L Pedersen
Jan 1, 1997·Cellular Signalling·J A Boutin
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Jan 16, 1999·The EMBO Journal·S M Fujihara, S G Nadler
Apr 28, 1999·Advances in Cancer Research·J S BiscardiS J Parsons
Dec 22, 1999·Seminars in Cell & Developmental Biology·J L Brodsky, A A McCracken
Dec 15, 2000·Oncogene·J D BjorgeD J Fujita
Jun 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A ChuU Stochaj
Aug 31, 2001·The Journal of Biological Chemistry·T A FaraziJ I Gordon
Mar 15, 2002·European Journal of Cell Biology·Frederic BardRoland Baron
Sep 17, 2003·The Journal of Biological Chemistry·Frédéric BardPierre Jurdic
Apr 3, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Scott M Dehm, Keith Bonham
May 25, 2004·Journal of Cellular Biochemistry·Ponniah SelvakumarRajendra K Sharma
Jun 2, 2004·Nature Reviews. Cancer·Timothy J Yeatman
Oct 19, 2004·Oncogene·G Steven Martin
Dec 8, 2004·Archives of Biochemistry and Biophysics·Matthew W VettingJohn S Blanchard
Mar 9, 2005·The Journal of Biological Chemistry·Shao H YangMartin O Bergo
Jul 27, 2005·Cell Biochemistry and Biophysics·Anuraag ShrivastavRajendra K Sharma
Jul 19, 2006·Progress in Lipid Research·Ponniah SelvakumarRajendra K Sharma

❮ Previous
Next ❯

Citations

Jan 1, 2014·Proteomics. Clinical Applications·Shweta BhatMahesh J Kulkarni
Oct 2, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Francesca RampoldiStefan Porubsky
Jul 18, 2017·Frontiers in Immunology·Daniel Ikenna UdenwobeleAnuraag Shrivastav
Aug 16, 2014·ChemMedChem·Can Zhao, Shutao Ma
Dec 30, 2014·Fundamental & Clinical Pharmacology·Amit KumarNirmal Singh
Jun 24, 2011·Cell and Tissue Research·Sujeet KumarRajendra K Sharma
Mar 19, 2021·Cellular & Molecular Immunology·Bin WangFangfang Zhou
Sep 29, 2009·Molecular and Biochemical Parasitology·Helen P PriceDeborah F Smith
Nov 9, 2010·Biochimie·Dale D O MartinLuc G Berthiaume

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.