Requirement of RNA synthesis for pathfinding by growing axons

The Journal of Comparative Neurology
R Von Bernhardi, M J Bastiani

Abstract

The effects of actinomycin D were studied in cultured grasshopper embryos at different stages of development by following the outgrowth patterns of identified neurones known as aCC, pCC, and Q1. When administered at stages occurring before 31% of embryonic development, actinomycin D (0.05-0.10 microM for 24-48 hours) prevented axon extension, whereas it did not affect the development of the nervous system in embryos older than 34% of development. At 31-34% of development, actinomycin D perturbed pathfinding of aCC without blocking axon extension. Thus, only 22% of the aCCs (n = 271) in embryos treated with actinomycin D extended an axon along the intersegmental nerve as in control embryos. In the remaining embryos, aCC failed to turn into the intersegmental nerve root; its growth cone remained in the longitudinal connective, above or below the turning point. Neurones of the group caudal to the intersegmental nerve root could extend along either the anterior or posterior commissure of the next posterior segment. In contrast to the observations made with aCC, only 1.2% of pCC (n = 166) and 0.0% of Q1 (n = 45) in embryos treated with actinomycin D showed axon growth along aberrant pathways. The position of the growth cones of most...Continue Reading

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Citations

Oct 4, 2002·Current Biology : CB·Barry Condron
Jul 18, 2001·Developmental Biology·R Shirasaki, F Murakami

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