Requirement of successive protein syntheses for the progress of meiosis in Blepharisma

Experimental Cell Research
E FriedlK Heckmann

Abstract

Germ nuclei of Blepharisma japonicum begin meiosis within a few hours when cells of complementary mating types conjugate. We synchronized the onset of conjugation and treated cells in different stages of meiosis with 10 micrograms/ml cycloheximide which strongly inhibits protein synthesis in this ciliate. Cycloheximide arrested meiosis at six stages: I, between pairing of cells and swelling of germ nuclei; II, leptotene; III, zygotene; IV, pachytene; XI, interkinesis; XII, prometaphase II. Five of these arrests were reversible. Puromycin (250-500 micrograms/ml) also inhibited the progress of meiosis, though to lesser extents. We propose that the progression of meiosis of B. japonicum requires at least six proteins which are synthesized sequentially during meiosis.

Citations

Aug 11, 2000·Micron : the International Research and Review Journal for Microscopy·G Santangelo, P Bruno
Feb 19, 1993·European Journal of Protistology·H W Kuhlmann, K Sato
Jan 1, 1987·Annals of the New York Academy of Sciences·D B Sanders

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