Requirement of TGFβ Signaling for Effect of Fluoride on Osteoblastic Differentiation

Biological Trace Element Research
Jingmin ZhangHui Xu

Abstract

Research focused on transforming growth factor β (TGFβ) signaling in osteoblast is gradually increasing, whereas literature is rare in terms of fluorosis. This work aimed to investigate how TGFβ signaling participated in regulation of the osteoblast by different doses of fluoride treatment. Bone marrow stem cells (BMSCs) were developed into osteoblastic cells and exposed to 1, 4, and 16 mg/L F- with and without 10 ng/mL of TGFβ. Cell viability and differentiation state of osteoblast under different settings were measured by means of cell counting kit and analysis of alkaline phosphatase (ALP) activity as well as formation of mineral nodules. Real-time PCR was utilized to test expression of ALP and Runt-related transcription factor 2 (Runx2) at gene level. The gene expression of TGFβ signaling effectors was also investigated, such as TGFβ receptors (TβRs), smad3, and mitogen-activated protein kinases (MAPK). Results demonstrated that fluoride treatment exhibited action on osteoblast viability and osteogenic differentiation and upregulated expression of TβR2, smad3, and MAPK in this process. Administration of TGFβ strengthened ALP activity but attenuated formation of mineral nodules. Co-treatment of TGFβ and low-dose fluoride inc...Continue Reading

References

Jul 1, 1994·Journal of Cellular Biochemistry·L F Bonewald, S L Dallas
May 25, 2010·Current Medicinal Chemistry·E GazzanoD Ghigo
May 26, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·David J J de GorterPeter ten Dijke
Feb 3, 2012·International Journal of Biological Sciences·Guiqian ChenYi-Ping Li
Jan 10, 2014·BoneKEy Reports·Simon Y Tang, Tamara Alliston
Nov 2, 2015·Methods in Molecular Biology·Janet L CraneXu Cao
May 17, 2017·Cold Spring Harbor Perspectives in Biology·Ingo GrafeYuji Mishina

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Citations

May 26, 2021·Biological Trace Element Research·Ningning JiangHui Xu

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

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