Requirement of the galU gene for polysaccharide production by and pathogenicity and growth In Planta of Xanthomonas citri subsp. citri.

Applied and Environmental Microbiology
Yinping GuoNian Wang

Abstract

Xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. In this study, we characterized the galU gene of X. citri subsp. citri. Two galU mutants (F6 and D12) were identified in an X. citri subsp. citri EZ-Tn5 <R6Kgammaori/KAN-2> Tnp transposon library. Rescue cloning, sequence analysis, and Southern blot analysis indicated that both of these mutants had a single copy of the EZ-Tn5 transposon inserted in galU in the chromosome. Further study showed that galU was required for biosynthesis of extracellular polysaccharides (EPS; xanthan gum) and capsular polysaccharide (CPS) and biofilm formation. Mutation of galU resulted in a loss of pathogenicity for grapefruit. The loss of pathogenicity of a galU mutant resulted from its inability to grow in planta rather than from the effect on virulence genes. Quantitative reverse transcription-PCR assays indicated that mutation of galU did not impair the expression of key virulence genes, such as pthA of X. citri subsp. citri. Although D12 had a growth rate similar to that of the wild-type strain in nutrient broth, no D12 population became established in the intercellular spaces of citrus leaves. Coinoculation of a galU mutant...Continue Reading

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Citations

Aug 9, 2013·BMC Microbiology·Tamara ZimaroNatalia Gottig
Mar 29, 2011·Annual Review of Phytopathology·Gwyn A Beattie
May 18, 2013·Molecular Plant-microbe Interactions : MPMI·N KillinyR P P Almeida
Jun 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li ShiDeyu Hu
May 27, 2016·Molecular Plant Pathology·Florencia A FicarraJorgelina Ottado
Apr 14, 2017·Journal of Basic Microbiology·Kulandaivelu VelmourouganeAnil Kumar Saxena
Jan 25, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Agustín ZavalaMarta Mollerach
Apr 14, 2018·Frontiers in Plant Science·Sunil Kumar SahuNan Yao
Jun 3, 2021·International Journal of Molecular Sciences·Natalia KaczynskaRobert Czajkowski
Aug 3, 2021·International Journal of Medical Microbiology : IJMM·Xin ZhaoYing Zhang

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