Requirements for budding of paramyxovirus simian virus 5 virus-like particles

Journal of Virology
Anthony P SchmittR A Lamb

Abstract

Enveloped viruses are released from infected cells after coalescence of viral components at cellular membranes and budding of membranes to release particles. For some negative-strand RNA viruses (e.g., vesicular stomatitis virus and Ebola virus), the viral matrix (M) protein contains all of the information needed for budding, since virus-like particles (VLPs) are efficiently released from cells when the M protein is expressed from cDNA. To investigate the requirements for budding of the paramyxovirus simian virus 5 (SV5), its M protein was expressed in mammalian cells, and it was found that SV5 M protein alone could not induce vesicle budding and was not secreted from cells. Coexpression of M protein with the viral hemagglutinin-neuraminidase (HN) or fusion (F) glycoproteins also failed to result in significant VLP release. It was found that M protein in the form of VLPs was only secreted from cells, with an efficiency comparable to authentic virus budding, when M protein was coexpressed with one of the two glycoproteins, HN or F, together with the nucleocapsid (NP) protein. The VLPs appeared similar morphologically to authentic virions by electron microscopy. CsCl density gradient centrifugation indicated that almost all of th...Continue Reading

References

Aug 1, 1992·Journal of Virology·M SuomalainenH Garoff
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Mar 1, 1994·Journal of Virology·S LopezJ H Strauss
Sep 1, 1995·Trends in Microbiology·J H StraussR J Kuhn
Sep 12, 2000·Journal of Virology·G Y Lin, R A Lamb
Oct 3, 2000·The EMBO Journal·K ForsellH Garoff
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·U SchubertJ W Yewdell
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·B StrackH G Gottlinger
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PatnaikJ W Wills
Nov 23, 2000·Journal of Virology·P Gómez-PuertasA Portela
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N HartyF P Hayes
Apr 21, 2001·Virology·J TimminsW Weissenhorn
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L VerPlankC A Carter

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Citations

Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Victoria A MoneyRobert P Yeo
Sep 15, 2006·Journal of Virology·Homer D PantuaTrudy G Morrison
Sep 29, 2006·Journal of Virology·Michael J Ciancanelli, Christopher F Basler
Feb 26, 2010·Journal of Virology·Lori W McGinnesTrudy G Morrison
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
May 6, 2011·Journal of Virology·Zhizhou KuangJonathan Leis
Jan 20, 2012·Journal of Virology·Megan S HarrisonAnthony P Schmitt
Jan 28, 2006·Journal of Virology·Rebecca L WilsonBiao He
Nov 13, 2008·Virology Journal·Jared R PatchChristopher C Broder
Apr 16, 2011·PloS One·Pramila WalpitaLinfa Wang
Nov 28, 2007·Uirusu·Takashi Irie, Takemasa Sakaguchi
Sep 21, 2010·Uirusu·Takashi Irie
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Jan 11, 2016·Wiley Interdisciplinary Reviews. RNA·Binbin DingMingzhou Chen

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