PMID: 6171868Jan 1, 1981Paper

Requirements for induction of specific suppressor T cells and detection of their H-2 antigen-binding receptors by fractionation on target cell monolayers

Scandinavian Journal of Immunology
B D BrondzZ K Blandova

Abstract

The MC-resistant specific suppressor T cells that inhibit DNA synthesis and CTL generation in MLC were induced in vivo by gamma-irradiated allogeneic lymphoid cells in a large dose. MLRs were inhibited only slightly when triggered by third-party cells, even neighbouring with the corresponding stimulators. Unlike the irradiated cells, intact allogeneic lymphoid cells induced a mixture of macrophage-like and T-cell suppressors with a pronounced non-specific component of the action. Syngeneic cells induced low active non-specific suppressors of macrophage type only. The suppression was not due to a cytotoxic effect, since specific T suppressors differed from CTL by conditions of induction and high sensitivity to gamma-irradiation and from CTL precursors by high sensitivity to CY and HC. The specific T-suppressors could be selectively removed by adherence to a macrophage monolayer of the corresponding donor. The subsequent elution of adherent lymphocytes with pronase resulted in enrichment of specific T suppressors by a factor of 30 and 2.6, as judged by reduction in the number of lymphocytes required for 50% inhibition of DNA synthesis and 33% inhibition of CTL generation, respectively. The high specificity of this enrichment is s...Continue Reading

References

Oct 1, 1979·Biulleten' eksperimental'noĭ biologii i meditsiny·A V KaraulovB D Brondz
Oct 1, 1978·The Journal of Experimental Medicine·G K Lewis, J W Goodman
Jan 1, 1979·Scandinavian Journal of Immunology·B D BrondzG I Drizlikh
Nov 1, 1977·Cellular Immunology·A EisenthalM Feldman
Mar 1, 1978·European Journal of Immunology·H N RodeJ Gordon
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C L ReinischS F Schlossman
Nov 1, 1977·The Journal of Experimental Medicine·M Taniguchi, J F Miller
Sep 15, 1978·Cellular Immunology·G Birnbaum, L Swick
Jun 1, 1977·Biulleten' eksperimental'noĭ biologii i meditsiny·B D BrondzA V Andreev
Jan 1, 1974·The Journal of Experimental Medicine·T Y HaH P Treffers
Mar 1, 1971·American Journal of Clinical Pathology·L T YamW H Crosby
Sep 1, 1964·The Journal of Experimental Medicine·A E REIF, J M ALLEN

❮ Previous
Next ❯

Citations

May 1, 1989·International Reviews of Immunology·H G Rammensee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.