Requirements for peptide binding to the human transporter associated with antigen processing revealed by peptide scans and complex peptide libraries.

The Journal of Biological Chemistry
S UebelR Tampé

Abstract

Antigenic peptides are translocated into the lumen of the endoplasmic reticulum by the action of the transporter associated with antigen processing (TAP), where they are subsequently needed for the correct assembly of major histocompatibility complex molecules. The transport function was reconstituted in insect cells by expression of both TAP genes. On the basis of this over-expression system, substrate selection was analyzed in detail by a direct biomolecular peptide binding assay. Competition assays with peptide variants, including substitutions of residues with alanine or structurally related amino acids, underline the broad peptide specificity of the human TAP complex. Steric requirements of the substrate-binding pocket were mapped using elongated peptides and scans with bulky, hydrophobic amino acids. Complex nonapeptide libraries were used to determine the contribution of each residue to stabilize peptide-TAP complexes. For the first time, this approach lets us directly evaluate the importance of peptide selection for the overall process of antigen presentation on the level of the peptide transporter.

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·M J AndrolewiczP Cresswell
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·T N SchumacherH L Ploegh
Feb 1, 1994·The Journal of Experimental Medicine·T N SchumacherH L Ploegh
Jul 1, 1993·Analytical Biochemistry·S Stevanović, G Jung

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Citations

May 20, 2006·Pflügers Archiv : European journal of physiology·Meike Herget, Robert Tampé
Aug 29, 2006·Pflügers Archiv : European journal of physiology·Kenneth J Linton, Christopher F Higgins
May 13, 2006·Journal of Bioenergetics and Biomembranes·Christian Schölz, Robert Tampé
Jul 16, 2013·Trends in Biochemical Sciences·Sabine Hulpke, Robert Tampé
Dec 10, 2002·Journal of Molecular Biology·Lars NeumannRobert Tampé
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Feb 1, 1996·Current Opinion in Immunology·P J Lehner, P Cresswell
Feb 1, 1996·Current Opinion in Immunology·P Walden
Feb 1, 1997·Current Opinion in Immunology·J O KoopmannF Momburg
May 14, 1999·Current Opinion in Immunology·S Uebel, R Tampé
Jun 7, 2012·Biochemistry·Andreas Hinz, Robert Tampé
Jul 21, 2010·Nature Chemical Biology·David Parcej, Robert Tampé
Sep 29, 2004·Nature Structural & Molecular Biology·Christopher F Higgins, Kenneth J Linton
Jan 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Meike HergetEnrica Bordignon
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chitra A ShintreElisabeth P Carpenter
Mar 2, 2011·The Journal of Biological Chemistry·Christian SchölzRobert Tampé
Jun 16, 2012·The Journal of Biological Chemistry·Valentina CorradiD Peter Tieleman
Jan 31, 2009·Antioxidants & Redox Signaling·Youngkyun KimKwangseog Ahn
Nov 3, 2007·The Journal of Clinical Investigation·Luc Teyton
Mar 15, 2006·BMC Bioinformatics·Irini A DoytchinovaDarren R Flower
Jan 4, 2006·PLoS Pathogens·Timothy Z VitalisWilfred A Jefferies
Aug 9, 2008·PLoS Pathogens·Youngkyun KimKwangseog Ahn
May 12, 2009·Biological Chemistry·Christian Schölz, Robert Tampé
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·S GorbulevR Tampé
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·S UebelR Tampé
Dec 20, 2005·FEBS Letters·Rupert Abele, Robert Tampé
Feb 4, 2014·Current Opinion in Chemical Biology·Ola Blixt, Ulrika Westerlind
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Sep 17, 2014·Journal of Molecular Biology·Peter U Mayerhofer, Robert Tampé
May 23, 1998·Annual Review of Immunology·E Pamer, P Cresswell
Jan 5, 2000·FEBS Letters·B Lankat-Buttgereit, R Tampé
Aug 1, 1996·European Journal of Immunology·J O KoopmannF Momburg
Jan 5, 2002·Physiological Reviews·Brigitte Lankat-Buttgereit, Robert Tampé
Apr 25, 2008·The Journal of Biological Chemistry·Chenguang ZhaoRupert Abele

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