Resampling and deconvolution of linear time-of-flight records for enhanced protein profiling.

Rapid Communications in Mass Spectrometry : RCM
Dariya I MalyarenkoDennis M Manos

Abstract

We have developed a peak deconvolution strategy that is applicable to the full mass range of a time-of-flight (TOF) spectrum. This strategy involves resampling a spectrum to create a time series that has equal peak widths (in time) across the entire spectrum, and then using the deconvolution filters we have previously described. We use this technique to deconvolve the protein mass spectra for blood serum and cell lysates acquired on three separate TOF instruments. Following deconvolution, we resolve spectral structures consistent with expected events such as multiply charged ions, matrix adducts and post-translational protein modifications. The deconvolution procedure produces a 40% improvement in the resolution and enhanced experimental sensitivity over the full length of the linear TOF record, up to m/z 150 000. This approach is particularly appropriate for automated data analysis and peak detection in dense TOF spectra.

References

Aug 15, 2003·Molecular & Cellular Proteomics : MCP·Radhakrishna S TirumalaiTimothy D Veenstra
Nov 20, 2003·Journal of the National Cancer Institute·David SidranskyLi Mao
Nov 9, 2004·European Journal of Mass Spectrometry·Jukka HeikkonenVirpi Tarkiainen
Jan 8, 2005·Molecular & Cellular Proteomics : MCP·Sheng PanRuedi Aebersold
Apr 26, 2006·Rapid Communications in Mass Spectrometry : RCM·Dariya I MalyarenkoDennis M Manos
Apr 1, 1995·Journal of the American Society for Mass Spectrometry·M W SenkoF W McLaffertycor

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Citations

Oct 9, 2007·Journal of Proteome Research·Christine L Gatlin-BunaiDariya I Malyarenko
Apr 10, 2010·BMC Bioinformatics·Karl W KuschnerEugene R Tracy
Oct 15, 2010·BMC Bioinformatics·Vincent A Emanuele, Brian M Gurbaxani
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Nov 4, 2010·Proteomics·Andrew W DowseyMichael J Dunn
Dec 5, 2009·Rapid Communications in Mass Spectrometry : RCM·Dariya I MalyarenkoDennis M Manos
Jul 16, 2016·Mass Spectrometry Reviews·Matthew B O'RourkeMatthew P Padula
Oct 5, 2007·Cancer Control : Journal of the Moffitt Cancer Center·Christine Laronga, Richard R Drake

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