PMID: 6990018May 1, 1980Paper

Rescue of abortive T7 gene 2 mutant phage infection by rifampin

Journal of Virology
M P Ontell, D Nakada

Abstract

Infection of Escherichia coli with T7 gene 2 mutant phage was abortive; concatemeric phage DNA was synthesized but was not packaged into the phage head, resulting in an accumulation of DNA species shorter in size than the phage genome, concomitant with an accumulation of phage head-related structures. Appearance of concatemeric T7 DNA in gene 2 mutant phage infection during onset of T7 DNA replication indicates that the product of gene 2 was required for proper processing or packaging of concatemer DNA rather than for the synthesis of T7 progeny DNA or concatemer formation. This abortive infection by gene 2 mutant phage could be rescued by rifampin. If rifampin was added at the onset of T7 DNA replication, concatemeric DNA molecules were properly packaged into phage heads, as evidenced by the production of infectious progeny phage. Since the gene 2 product acts as a specific inhibitor of E. coli RNA polymerase by preventing the enzyme from binding T7 DNA, uninhibited E. coli RNA polymerase in gene 2 mutant phage-infected cells interacts with concatemeric T7 DNA and perturbs proper DNA processing unless another inhibitor of the enzyme (rifampin) was added. Therefore, the involvement of gene 2 protein in T7 DNA processing may be ...Continue Reading

References

Dec 1, 1977·Journal of Virology·B A Hesselbach, D Nakada
Oct 15, 1978·Journal of Molecular Biology·S Strome, E T Young
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E LeClerc, C C Richardson
Apr 15, 1976·Nature·R S Hayward, J G Scaife
Dec 1, 1975·Journal of Virology·Y YamadaD Nakada
Jul 1, 1975·Journal of Virology·M S Center
Jul 21, 1972·Journal of Molecular Biology·R A Schlegel, C A Thomas
Sep 28, 1973·Nature·W Strätling, R Knippers
Mar 22, 1974·Nature·Y YamadaD Nakada
Jun 27, 1974·Molecular & General Genetics : MGG·D H Boyd, W Zillig
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Nov 1, 1969·Virology·F W Studier
Nov 1, 1969·Virology·F W Studier, J V Maizel
Jan 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M S CenterC C Richardson

❮ Previous
Next ❯

Citations

Oct 7, 2003·Annual Review of Microbiology·Sergei Nechaev, Konstantin Severinov
Jan 1, 1982·Molecular & General Genetics : MGG·S R Buchstein, D C Hinkle
Jun 17, 1999·Journal of Molecular Biology·S Nechaev, K Severinov
Mar 1, 1981·Microbiological Reviews·D H Krüger, C Schroeder
Jul 10, 2003·Journal of Molecular Biology·Julia YuzenkovaKonstantin Severinov
Jun 5, 1981·Journal of Molecular Biology·J J Dunn, F W Studier
Jul 24, 2010·Journal of Molecular Biology·Dhruti SavaliaKonstantin Severinov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.