PMID: 9535220Apr 16, 1998Paper

Rescue of cells from apoptosis by inhibition of active GSH extrusion

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Lina GhibelliM R Ciriolo

Abstract

Cells induced to apoptosis extrude glutathione in the reduced form concomitantly with (U937 cells) or before (HepG2 cells) the development of apoptosis, much earlier than plasma membrane leakage. Two specific inhibitors of carrier-mediated GSH extrusion, methionine or cystathionine, are able to decrease apoptotic GSH efflux across the intact plasma membrane, demonstrating that in these cell systems GSH extrusion occurs via a specific mechanism. While decreasing GSH efflux, cystathionine or methionine also decrease the extent of apoptosis. They fail to exert anti-apoptotic activity in cells previously deprived of GSH, indicating that the target of the protection is indeed GSH efflux. The cells rescued by methionine or cystathionine remained viable after removal of the apoptogenic inducers and were even able to replicate. This shows that a real rescue to perfect viability and not just a delay of apoptosis is achieved by forcing GSH to stay within the cells during apoptogenic treatment. All this evidence indicates that extrusion of reduced glutathione precedes and is responsible for the irreversible morphofunctional changes of apoptosis, probably by altering the intracellular redox state without intervention of reactive oxygen spe...Continue Reading

References

Jan 1, 1990·Annual Review of Pharmacology and Toxicology·D J Reed
Dec 1, 1987·The Journal of Pathology·A H Wyllie
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 1, 1980·Archives of Biochemistry and Biophysics·P W Beatty, D J Reed
Nov 2, 1995·Biochemical and Biophysical Research Communications·L GhibelliM R Ciriolo
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Apr 27, 1995·Nature·M D Jacobson, M C Raff
May 1, 1995·Trends in Biochemical Sciences·S Kumar
Apr 27, 1995·Nature·S ShimizuY Tsujimoto
Jan 1, 1995·Experimental Cell Research·S C ChowS Orrenius
Jan 1, 1994·Immunology Today·T M Buttke, P A Sandstrom
Mar 15, 1996·Experimental Cell Research·L DiniL Ghibelli
Jun 28, 1996·The Journal of Biological Chemistry·D J van den DobbelsteenA F Slater

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Citations

Apr 15, 1999·European Journal of Clinical Investigation·A G Hall
Oct 29, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C ColussiL Ghibelli
Jul 14, 2001·Redox Report : Communications in Free Radical Research·R J Carmody, T G Cotter
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erin K Pias, Tak Yee Aw
Aug 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lina MusallamMarc Bilodeau
Jun 17, 2003·Molecular and Cellular Biology·Linyun ChenJefferson Y Chan
Aug 26, 2003·Redox Report : Communications in Free Radical Research·Bengt Fadeel, Valerian E Kagan
Mar 8, 2007·Annals of the New York Academy of Sciences·Silvia CristofanonLina Ghibelli
Mar 21, 2007·Biochemistry. Biokhimii︠a︡·O N Oktyabrsky, G V Smirnova
Mar 27, 2007·Annals of the New York Academy of Sciences·M De NicolaL Ghibelli
Dec 26, 2007·Archives of Physiology and Biochemistry·R FrancoM I Panayiotidis
Aug 29, 2007·The Journal of Biological Chemistry·Rodrigo FrancoJohn A Cidlowski
May 29, 2009·Archives of Toxicology·Tomoyuki Sumizawa, Hideki Igisu
Sep 30, 2010·The Journal of Pharmacology and Experimental Therapeutics·Prasun GuhaSandip K Bandyopadhyay
Nov 14, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mette E Skindersoe, Soeren Kjaerulff
Feb 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Priya Kalyan GopalSantanu Paul
Sep 11, 2014·Frontiers in Pharmacology·Katia AquilanoMaria R Ciriolo
Aug 1, 2015·Cardiovascular Research·Julio Madrigal-MatuteLuis Miguel Blanco-Colio
Jul 12, 2016·Metallomics : Integrated Biometal Science·M A GreenoughC M Opazo
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Xiuli WangHongfang Jin
Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G LizardP Gambert
Dec 29, 2000·Current Opinion in Cardiology·J S Forrester
Dec 23, 2000·The Journal of Urology·R N CoffeyJ M Fitzpatrick
Dec 28, 2002·The Journal of Biological Chemistry·Yu-Ying HeColin F Chignell
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C FanelliL Ghibelli
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H HentzeA Wendel
Sep 29, 1999·Journal of Neurochemistry·C WallinM Sandberg
Oct 30, 2003·Journal of Cellular Physiology·Lina MusallamMarc Bilodeau
Aug 27, 1999·Scandinavian Journal of Clinical and Laboratory Investigation·T FoulonA Hadjian
Jun 24, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M D'AlessioL Ghibelli
Sep 6, 2003·Biological Chemistry·Yong-Won KwonJunji Yodoi
Mar 23, 2004·Annals of the New York Academy of Sciences·Francesca LiuzziLina Ghibelli
Mar 11, 2006·Apoptosis : an International Journal on Programmed Cell Death·L GhibelliL M Porfiri
Jan 16, 2007·FEBS Letters·Milena HristovaAlbert van der Vliet
Jul 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Erik I FinkelsteinAlbert van der Vliet
Jan 1, 2010·Journal of Medicinal Food·Andriana C KalioraVaios T Karathanos

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