PMID: 9434157Jan 20, 1998Paper

Rescue of polyomavirus DNA after co-transfection of recombinant plasmids with viral DNA fragments

Biochimica Et Biophysica Acta
L C FortierP Bourgaux

Abstract

Plasmid DNA bearing a single copy of the mouse polyomavirus (Py) genome (template A) was transfected into murine cells together with another DNA (template B) carrying intact the viral sequence interrupted in template A. Rescue of unit-length Py DNA including markers from both templates was observed as long as the viral DNA in B overlapped that split in A by one kbp or more. Such rescue was not detectably enhanced by linearizing either or both template(s), and occurred in the absence of template replication. These findings are suggestive of an intermolecular recombination process taking place soon after transfection and starting with homologous pairing between A and B. Such pairing would facilitate removal of vector DNA from one template (A), followed by closure of the resulting break or gap through recombination with the other template (B). Since B may consist of a PCR-synthesized DNA fragment, these observations could conceivably serve as the basis for a method of generating mutant viral genomes.

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M S Meselson, C M Radding
May 1, 1990·Journal of Virology·P BourgauxD Bourgaux-Ramoisy
Jan 1, 1989·Annual Review of Genetics·R J BollagR M Liskay
Mar 1, 1985·Molecular and Cellular Biology·J Rubnitz, S Subramani
Jan 1, 1985·Molecular & General Genetics : MGG·H Kovar, E Wintersberger
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D A BrennerR D Camerini-Otero
Jul 1, 1986·Molecular and Cellular Biology·S Chakrabarti, M M Seidman
Mar 1, 1987·Journal of Virology·A Piché, P Bourgaux
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D AyaresR Kucherlapati
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Aug 1, 1967·Biochemistry·D B Millar, M MacKenzie
Aug 1, 1984·Molecular and Cellular Biology·D J Sussman, G Milman
Nov 1, 1984·Molecular and Cellular Biology·J Rubnitz, S Subramani
Oct 15, 1981·Experientia·C GélinasM Bastin
Jan 1, 1982·Annual Review of Genetics·C M Radding
Jun 1, 1983·Molecular and Cellular Biology·S Subramani, P Berg
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R S KucherlapatiO Smithies
May 1, 1983·Cell·J W SzostakF W Stahl
Jan 10, 1980·Nature·A J Twigg, D Sherratt
May 1, 1994·Mutation Research·A Belmaaza, P Chartrand
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·D J Segal, D Carroll
Jul 1, 1996·Journal of Virology·D GendronP Bourgaux
Jun 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Federico CappuzzoMarileila Varella-Garcia

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