Rescue of recent virulent and avirulent field strains of bluetongue virus by reverse genetics.

PloS One
René G P van GennipPiet A van Rijn

Abstract

Since 1998, Bluetongue virus (BTV)-serotypes 1, 2, 4, 9, and 16 have invaded European countries around the Mediterranean Basin. In 2006, a huge BT-outbreak started after incursion of BTV-serotype 8 (BTV8) in North-Western Europe. More recently, BTV6 and BTV11 were reported in North-Western Europe in 2008. These latter strains are closely related to live-attenuated vaccine, whereas BTV8 is virulent and can induce severe disease in ruminants, including cattle. In addition, Toggenburg orbivirus (TOV) was detected in 2008 in Swiss goats, which was recognized as a new serotype of BTV (BTV25). The (re-)emergency of known and unknown BTV-serotypes needs a rapid response to supply effective vaccines, and research to study this phenomenon. Recently, orbivirus research achieved an important breakthrough by the establishment of reverse genetics for BTV1. Here, reverse genetics for two recent BTV strains representing virulent BTV8 and avirulent BTV6 was developed. For this purpose, extensive sequencing of full-genomes was performed, resulting in the consensus sequences of BTV8/net07 and BTV6/net08. The recovery of 'synthetic BTV', respectively rgBTV8 and rgBTV6, completely from T7-derived RNA transcripts was confirmed by silent mutations b...Continue Reading

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Oct 12, 2010·Virology Journal·René G P van GennipPiet A van Rijn

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Citations

Jun 19, 2015·Vector Borne and Zoonotic Diseases·Barbara S DroletPeter P Mertens
Apr 26, 2016·Vector Borne and Zoonotic Diseases·Barbara S DroletRichard A Bowen
Jan 28, 2014·PloS One·René G P van GennipPiet A van Rijn
Dec 3, 2016·Journal of Virology·René G P van GennipPiet A van Rijn
Oct 13, 2019·Parasites & Vectors·René G P van GennipPiet A van Rijn
Dec 12, 2019·Frontiers in Veterinary Science·Piet A van Rijn

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Methods Mentioned

BETA
genetic modification
transfection
electrophoresis
ELISA

Software Mentioned

Lasergene
Potgieter
Seqman

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