PMID: 8995650Feb 1, 1997Paper

Rescue of rinderpest virus from cloned cDNA

Journal of Virology
M D Baron, T Barrett

Abstract

Rinderpest virus is a morbillivirus and is the causative agent of a widespread and important disease of cattle. The viral genome is a single strand of RNA in the negative sense. We have constructed plasmids containing cDNA copies of the 5' and 3' termini of the virus separated by a reporter gene and have shown that antigenome-sense RNA transcripts of these model genomes can be replicated, transcribed, and packaged by helper virus, both rinderpest virus and the related measles virus. Further, these genome analogs can be replicated and transcribed by viral proteins expressed from cDNA clones by using a recombinant vaccinia virus expressing T7 RNA polymerase (MVA-T7). Using this latter system, we have rescued live rinderpest virus from a full-length cDNA copy of the genome of the RBOK vaccine strain. The recombinant virus appears to grow in tissue culture identically to the original virus.

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Citations

Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·A PekoszR A Lamb
Aug 31, 1999·The Journal of General Virology·M D BaronT Barrett
Nov 21, 2001·The Journal of General Virology·V MiouletM D Baron
Nov 12, 2003·Journal of Virology·Veronika von MesslingRoberto Cattaneo
Nov 19, 2011·Biotechnology and Bioengineering·Liping DuAnthony C Forster
Sep 17, 2014·Veterinary Research Communications·Fuxiao LiuZhiliang Wang
Oct 21, 1999·Microbiology and Immunology·Y Nagai, A Kato
Dec 2, 1999·The Journal of General Virology·A Römer-OberdörferT C Mettenleiter
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Mar 24, 2005·The Journal of General Virology·Ashley C BanyardThomas Barrett
Aug 4, 2009·Biotechnology and Bioengineering·Liping DuAnthony C Forster
Feb 22, 2013·The Journal of General Virology·Jana Baron, Michael Baron
Mar 24, 2005·The Journal of General Virology·M D BaronT Barrett
Oct 24, 2000·Journal of Virology·U GassenB K Rima
Jan 10, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M HattaY Kawaoka
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G NeumannY Kawaoka
Mar 11, 2005·Expert Review of Vaccines·Thomas Barrett
Jul 16, 2004·Journal of Virology·Sander HerfstRon A M Fouchier
Apr 14, 2017·MSphere·Shannon M BeatyBenhur Lee
May 26, 2005·The Journal of General Virology·Michael D Baron
Jun 23, 2000·Journal of Virology·M TakedaM Tashiro
Jun 20, 2020·Science·Ariane DüxSébastien Calvignac-Spencer

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