Rescuing an essential enzyme-RNA complex with a non-essential appended domain

The EMBO Journal
E F Whelihan, P Schimmel

Abstract

Certain protein-RNA complexes, such as synthetase-tRNA complexes, are essential for cell survival. These complexes are formed with a precise molecular fit along the interface of the reacting partners, and mutational analyses have shown that amino acid or nucleotide substitutions at the interface can be used to disrupt functional or repair non-functional complexes. In contrast, we demonstrate here a feature of a eukaryote system that rescues a disrupted complex without directly re-engineering the interface. The monomeric yeast Saccharomyces cerevisiae glutaminyl-tRNA synthetase, like several other class I eukaryote tRNA synthetases, has an active-site-containing 'body' that is closely homologous to its Escherichia coli relative, but is tagged at its N-terminus with a novel and dispensable appended domain whose role has been obscure. Because of differences between the yeast and E. coli glutamine tRNAs that presumably perturb the enzyme-tRNA interface, E. coli glutaminyl-tRNA synthetase does not charge yeast tRNA. However, linking the novel appended domain of the yeast to the E. coli enzyme enabled the E. coli protein to function as a yeast enzyme, in vitro and in vivo. The appended domain appears to contribute an RNA interaction ...Continue Reading

References

Dec 20, 1986·Journal of Molecular Biology·W A Weiss, E C Friedberg
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D M CrothersG Söll
Jul 1, 1984·Cell·I A WilsonR A Lerner
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H InokuchiD Söll
Jun 30, 1995·Cell·P Schimmel, L Ribas de Pouplana
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·V LamourM Mirande
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·S B RhoS Kim

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Citations

Dec 16, 2004·Angewandte Chemie·Lei Wang, Peter G Schultz
Sep 3, 2002·FEMS Microbiology Letters·Sanda RocakIvana Weygand-Durasevic
Sep 1, 2011·ACS Chemical Biology·Shun-Jia ChenChien-Chia Wang
Nov 26, 2010·The Journal of Biological Chemistry·Vlatka Godinic-MikulcicIvana Weygand-Durasevic
May 27, 2005·The Journal of Biological Chemistry·Mette Praetorius-IbbaMichael Ibba
Aug 30, 2008·The Journal of Biological Chemistry·Chia-Pei ChangChien-Chia Wang
Nov 4, 1998·Nucleic Acids Research·R GiegéC Florentz
Dec 20, 2011·Nucleic Acids Research·Thomas D GrantElizabeth J Grayhack
Jul 24, 2012·Nucleic Acids Research·Chih-Chi LiaoChien-Chia Wang
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·J W Chihade, P Schimmel
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·S B RhoK Shiba
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·A K KowalU L RajBhandary
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·D R Liu, P G Schultz
Aug 26, 2014·Journal of Molecular Biology·Andrew Hadd, John J Perona
Aug 31, 2000·Annual Review of Biochemistry·M Ibba, D Soll
May 29, 1999·The Journal of Biological Chemistry·C C Wang, P Schimmel
Jun 8, 1999·The Journal of Biological Chemistry·S G ParkK Shiba
Apr 4, 2000·FEBS Letters·E Berthonneau, M Mirande
Dec 18, 2002·The Journal of Biological Chemistry·Michaël RyckelynckMagali Frugier
Apr 5, 2000·The Journal of Biological Chemistry·C C WangP Schimmel
Jan 26, 2005·Biochemistry·Jean-Sébastien GraindorgeFranco Fasiolo
Oct 22, 2021·Journal of Molecular Biology·Sooin KimHyun Soo Lee

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