Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells

Genetics and Molecular Research : GMR
R F LiuK M Zhang

Abstract

Psoriasis is an inflammatory skin disease characterized by excessive proliferation and abnormal differentiation and apoptosis of keratinocytes (KCs). Mesenchymal stem cells (MSCs) from skin lesions of psoriasis patients demonstrate abnormal cytokine secretion, which may affect KC proliferation and apoptosis. Here, we explored how MSCs from skin lesions of psoriasis patients affect HaCaT cell proliferation and apoptosis. First, flow cytometry and multipotent differentiation methods were used to identify skin MSCs, which were then co-cultured with HaCaT cells. HaCaT cell proliferation was analyzed in real-time, and cell cycle progression and apoptosis were assessed by flow cytometry. Cell morphologies and multipotencies of skin MSCs were similar between the psoriasis group and healthy control group, with high levels of CD29, CD44, CD73, CD90, and CD105 and limited expression of CD34, CD45, and HLA-DR. MSCs from skin lesions of psoriasis patients promote KC proliferation more potently and are less capable of inducing KC apoptosis. This may underlie KC proliferation and abnormal apoptosis in psoriasis skin lesions, which results in abnormal thickening of the epidermis.

Citations

Oct 21, 2017·International Journal of Molecular Sciences·Agnieszka Owczarczyk-SaczonekJoanna Wojtkiewicz
Jan 4, 2018·Marine Drugs·Azahara Rodríguez-LunaJavier Ávila-Román
Sep 11, 2018·International Journal of Dermatology·Qiang WangKaiming Zhang
May 13, 2020·The Journal of Dermatology·Xinhua LiKaiming Zhang
May 10, 2020·Clinical and Experimental Dermatology·A PaganelliC Magnoni
Aug 3, 2019·International Journal of Molecular Sciences·Francisco J VizosoRoman Perez-Fernandez
Jan 29, 2021·Stem Cells International·Yanyun LiJincheng Zeng
May 28, 2021·Life Sciences·Deepak Hiraganahalli Bhaskarmurthy, Sabina Evan Prince
Aug 27, 2021·International Journal of Dermatology·Yue CaoKai-Ming Zhang

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