Jun 17, 1998

Research notes: Fresh and frozen pools of chicken red blood cells as substrates for direct polymerase chain reaction

Poultry Science
H KhatibY Gruenbaum

Abstract

A method is presented for reliable use of pooled chicken blood samples for estimation of microsatellite frequencies by direct polymerase chain reaction (PCR) amplification of DNA. This method overcomes the variability of hematocrit values in individual chickens and eliminates the step of DNA preparation. The estimated frequencies of polymorphic alleles in fresh and frozen pooled blood samples were similar to those obtained by calculating these frequencies from the individual genotyping. When frozen pooled blood samples are used, pools should be prepared prior to their freezing.

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Mentioned in this Paper

Short Tandem Repeat
Collection of Blood Specimen for Laboratory Procedure
Blood Preservation
Red blood cells, blood product
Polymerase Chain Reaction Analysis
Gene Amplification Technique
Genome Mapping
Quantitative Trait, Heritable
Red Blood Cell Count Measurement
Cryonic Suspension

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