PMID: 22568333May 10, 2012Paper

Research of histochemical staining for identifying the function and morphology of fascicles in three-dimensional reconstruction of peripheral nerves

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Peng LuoXiaolin Liu

Abstract

To explore the histochemical staining for distinguishing and localizing nerve fibers and fascicles at histological level in three-dimensional reconstruction of peripheral nerves. The right median nerve was harvested from one fresh cadaver and embedded in OCT compound. The sample was serially horizontally sliced with 6 microm thickness. All sections were stained with Karnovsky-Roots method (group A, n = 30) firstly and then stained with toluidine blue (group B, n = 28) and Ponceau 2R (group C, n = 21) in proper sequence. The results of each step were taken photos (x 100). After successfully stitching, the two-dimensional panorama images were compared, including texture feature, the number and aver gray level of area showing acetylcholinesterase (AchE) activity, and result of auto microscopic medical image segmentation. In groups A, B, and C, the number of AchE-positive area was (21.63 +/- 4.06) x 10(2), (20.64 +/- 3.51) x 10(2), and (20.54 +/- 5.71) x 10(2), respectively, showing no significant difference among 3 groups (F = 0.64, P = 0.54); the mean gray level was (1.41 +/- 0.06) x 10(2), (1.10 +/- 0.05) x 10(2), and (1.14 +/- 0.07) x 10(2), respectively, showing significant differences between group A and groups B and C (P < 0...Continue Reading

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