PMID: 19137817Jan 14, 2009Paper

Research on differentiation features of the monoclonal human pancreatic stem cell

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology
Mei XiaoZhongying Dou

Abstract

The in vitro and in vivo differentiation features of the monoclonal human pancreatic stem cell (mhPSC) line derived from the pancreatic tissues of a male abortive fetus at 4 month-old were studied. The mhPSCs were plated in culture dishes that had been coated with 0.1% gelatin in phosphate-buffered saline without calcium and magnesium. After proliferated for 3 days, the mhPSCs were induced in modified high-glucose Dulbecco's Modified Eagles's Medium for 25 days. The changes of the cell morphology were observed by phasecontrast microscope during inducement course. The results of the mhPSCs in vitro induced to differentiate into functional pancreatic islets were identified using dithizone staining, RT-PCR and stimulation-glucose secreting insulin and C-peptide radioimmunoassay. The mhPSCs suspension was separately injected under the groin hypoderm of male nude mice. On the 30th day, the grafts were taken off. The immunochemistry reactions were performed by the SP method. The in vivo differentiation ability of the mhPSCs in nude mice was assessed. In vitro proliferation culture, the mhPSCs adhesively grew and showed polygon epithelioid morphology. After proliferation a layer, the mhPSCs showed the gravelstone-like. During in vitro...Continue Reading

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