Research Resource: A Chromogranin A Reporter for Serotonin and Histamine Secreting Enteroendocrine Cells

Molecular Endocrinology
Maja S EngelstoftThue W Schwartz

Abstract

Chromogranin A (ChgA) is an acidic protein found in large dense-core secretory vesicles and generally considered to be expressed in all enteroendocrine cells of the gastrointestinal (GI) tract. Here, we characterize a novel reporter mouse for ChgA, ChgA-humanized Renilla reniformis (hr)GFP. The hrGFP reporter was found in the monoamine-storing chromaffin cells of the adrenal medulla, where ChgA was originally discovered. hrGFP also was expressed in enteroendocrine cells throughout the GI tract, faithfully after the expression of ChgA, as characterized by immunohistochemistry and quantitative PCR analysis of fluorescence-activated cell sorting-purified cells, although the expression in the small intestine was weak compared with that of the stomach and colon. In the stomach, hrGFP was highly expressed in almost all histamine-storing enterochromaffin (EC)-like cells, at a lower level in the majority of serotonin-storing EC cells and ghrelin cells, in a small fraction of somatostatin cells, but was absent from gastrin cells. In the small intestine, the hrGFP reporter was selectively, but weakly expressed in EC cells, although not in any peptide-storing enteroendocrine cells. In the colon, hrGFP was exclusively expressed in EC cells...Continue Reading

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Citations

Jul 23, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael CamilleriJoseph A Murray
Sep 29, 2017·Diabetologia·Tina JorsalFilip K Knop
Aug 26, 2018·Development·Katie L SinagogaJames M Wells
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Feb 11, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kunrong ChengJean-Pierre Raufman
May 4, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Kang ZhouZhenshan Wang
Mar 19, 2021·The Journal of Veterinary Medical Science·Kimie Niimi, Eiki Takahashi
Aug 28, 2021·Stem Cell Reports·Sina MohammadiLinda A Lieberman
Oct 25, 2021·Gastroenterology·Anthony J TreichelArthur Beyder

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