Research Techniques Made Simple: Techniques to Assess Cell Proliferation

The Journal of Investigative Dermatology
George A RomarSherrie J Divito

Abstract

Cell proliferation is commonly assayed in the laboratory for research purposes, but is increasingly used clinically to gauge tumor aggressiveness and potentially guide care. Therefore, both researchers and clinicians should have a basic understanding of techniques used to assess cell proliferation. Multiple cell proliferation assays exist, and the choice of method depends on the laboratory resources available, the types of cells/tissues to be studied, and the specific experimental goals. In this article, we identify four overarching categories of cell proliferation assays that signify various stages of the cell cycle: nucleoside-analog incorporation, cell cycle-associated protein detection, use of cytoplasmic proliferation dyes, and indirect measures of cell proliferation. Each method has strengths and limitations that should guide the dermatology investigator's choice of assay.

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Citations

Dec 12, 2019·Anti-cancer Agents in Medicinal Chemistry·Deepa NegiNeeraj Mishra
Aug 17, 2018·Marine Drugs·Olga Maria LageFrancisca Vicente
Oct 19, 2019·Cell Division·Tatiana G Ruksha
Jul 8, 2020·BMC Veterinary Research·Karla Lucía F AlvarezManolo Fernández-Díaz
Jan 16, 2019·Nanomaterials·Daniel Mihai TeleanuRaluca Ioana Teleanu
Oct 12, 2018·Scientific Reports·Nicholas R HumGabriela G Loots
Jun 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Piotr MajWojciech Rode
Jun 16, 2019·Bulletin of Mathematical Biology·Ana Victoria Ponce BobadillaTomás Alarcón
Nov 22, 2020·Journal of Dentistry·Qin SunFranklin R Tay
Nov 19, 2020·Food & Function·Sara Shojaei-ZarghaniSaber Azami-Aghdash
Mar 23, 2021·Journal of Immunological Methods·Bernhardt SachsAndreas Glässner
Jun 3, 2021·Current Issues in Molecular Biology·Egidijus ŠimoliūnasDaiva Baltriukienė
Nov 12, 2021·Current Protocols·Mohini SinghPriscilla K Brastianos

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