Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis.

International Journal of Molecular Sciences
Anna Maria Wójcik

Abstract

During early plant embryogenesis, some of the most fundamental decisions on fate and identity are taken making it a fascinating process to study. It is no surprise that higher plant embryogenesis was intensively analysed during the last century, while somatic embryogenesis is probably the most studied regeneration model. Encoded by the MIRNA, short, single-stranded, non-coding miRNAs, are commonly present in all Eukaryotic genomes and are involved in the regulation of the gene expression during the essential developmental processes such as plant morphogenesis, hormone signaling, and developmental phase transition. During the last few years dedicated to miRNAs, analytical methods and tools have been developed, which have afforded new opportunities in functional analyses of plant miRNAs, including (i) databases for in silico analysis; (ii) miRNAs detection and expression approaches; (iii) reporter and sensor lines for a spatio-temporal analysis of the miRNA-target interactions; (iv) in situ hybridisation protocols; (v) artificial miRNAs; (vi) MIM and STTM lines to inhibit miRNA activity, and (vii) the target genes resistant to miRNA. Here, we attempted to summarise the toolbox for functional analysis of miRNAs during plant embryo...Continue Reading

References

Jul 9, 2002·Genes & Development·Brenda J ReinhartDavid P Bartel
May 3, 2003·Current Biology : CB·Zhixin XieJames C Carrington
Apr 22, 2004·The Plant Cell·Thomas LauxHolger Breuninger
Jun 18, 2004·Molecular Cell·Matthew W Jones-Rhoades, David P Bartel
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel
Jul 11, 2006·The Plant Journal : for Cell and Molecular Biology·Anna VálócziZoltán Havelda
Jul 24, 2007·Nature Genetics·José Manuel Franco-ZorrillaJavier Paz-Ares
Aug 4, 2007·The Plant Journal : for Cell and Molecular Biology·Po-Pu LiuJames C Carrington
Oct 13, 2007·Plant Methods·Erika Varkonyi-GasicRoger P Hellens
Feb 14, 2008·The Plant Journal : for Cell and Molecular Biology·Stephan OssowskiDetlef Weigel
Feb 16, 2008·Nature Protocols·Eva VárallyayZoltán Havelda
Apr 18, 2008·BMC Plant Biology·Ramanjulu Sunkar, Guru Jagadeeswaran
Aug 19, 2008·Seminars in Cell & Developmental Biology·Damien Garcia
Aug 8, 2009·BMC Genomics·Wuttichai Mhuantong, Duangdao Wichadakul
Mar 17, 2010·Developmental Cell·Kaoru SugimotoElliot M Meyerowitz
Aug 20, 2010·Nucleic Acids Research·Yijun MengMing Chen
Oct 20, 2010·International Journal of Molecular Sciences·Xiaosu WangShenghua Wang
Feb 19, 2011·Plant Physiology·Matthew R WillmannPablo D Jenik
May 12, 2011·Cardiovascular Research·Minna U KaikkonenChristopher K Glass
Feb 6, 2013·Journal of Experimental Botany·Xiyan YangXianlong Zhang
Jun 4, 2013·Trends in Plant Science·Joakim PalovaaraDolf Weijers

❮ Previous
Next ❯

Citations

Aug 13, 2020·International Journal of Molecular Sciences·Robert Hasterok, Alexander Betekhtin

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
RNA-seq
reverse transcription PCR
sRNA-seq
FANS

Software Mentioned

TransmiR
SE
AmiRNA Designer Small RNA Maker Suite SAMS
tools4miRs
miRVIT
MepmiRDB
Web MicroRNA Designer
miRbase
ZE
INTACT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.