Reshaping and Customization of SMILE-Derived Biological Lenticules for Intrastromal Implantation

Investigative Ophthalmology & Visual Science
Iben Bach DamgaardJodhbir Singh Mehta

Abstract

To evaluate the feasibility of excimer laser reshaping of biological lenticules available after small incision lenticule extraction (SMILE). Fresh and cryopreserved SMILE-derived human lenticules underwent excimer laser ablation for stromal reshaping. The treatment effects in the lasered group were compared with the nonlasered group with respect to changes in surface functional groups (by Fourier transform infrared spectroscopy [FTIR]) and surface morphology (by scanning electron microscopy [SEM] and atomic force microscopy [AFM]). Ten SMILE-derived porcine lenticules, five nonlasered (107-μm thick, -6 diopter [D] spherical power) and five excimer lasered (50% thickness reduction), were implanted into a 120-μm stromal pocket of 10 porcine eyes. Corneal thickness and topography were assessed before and after implantation. FTIR illustrated prominent changes in the lipid profile. The collagen structure was also affected by the laser treatment but to a lesser extent. SEM exhibited a more regular surface for the lasered lenticules, confirmed by the lower mean Rz value (290.1 ± 96.1 nm vs. 380.9 ± 92.6 nm, P = 0.045) on AFM. The lasered porcine lenticules were thinner than the nonlasered controls during overhydration (132 ± 26 μm vs....Continue Reading

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Citations

Jul 6, 2020·Science China. Life Sciences·Ye ShenJianwu Dai
Oct 23, 2019·Investigative Ophthalmology & Visual Science·Francisco BandeiraJodhbir S Mehta
Sep 8, 2019·Progress in Retinal and Eye Research·Andri K RiauJodhbir S Mehta
Feb 2, 2021·Experimental Eye Research·Beau J FennerJodhbir S Mehta
Feb 21, 2021·International Ophthalmology·Andri K RiauJodhbir S Mehta
Feb 25, 2021·International Ophthalmology·Adriano FasoloGiorgio Marchini

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