Residence at the expression site is necessary and sufficient for the transcription of surface antigen genes of Pneumocystis carinii

Molecular Microbiology
S M Sunkin, J R Stringer

Abstract

The major surface glycoprotein (MSG) of P. carinii f. sp. carinii is a family of proteins encoded by a family of heterogeneous genes. Messenger RNAs encoding different MSG isoforms start with the same sequence, called the upstream conserved sequence (UCS), which is encoded by a single locus. The mechanism by which the UCS becomes part of different MSG mRNAs is not obvious because at least 15 loci, which are distributed throughout the genome, encode MSGs. One possibility is that attachment to the UCS locus is required for the transcription of an MSG gene. The alternative to this expression site model is that mRNAs acquire the UCS by RNA splicing. To distinguish between these two models, UCS/MSG junctions in the genome were compared with UCS/MSG junctions in mRNA. The UCS/MSG junctions in the mRNA matched those in the genome, as would be expected if splicing did not contribute to the attachment of the UCS to the 5' ends of MSG mRNAs. Given that few if any MSG mRNAs lack the UCS, the correspondence between the UCS/MSG junctions in transcripts and those in the genome indicates that attachment to the UCS is both necessary and sufficient for transcription of an MSG gene.

Citations

Jun 9, 2009·Nature Reviews. Microbiology·Kirk W DeitschJames R Stringer
Jul 17, 2008·The Journal of Infectious Diseases·Geetha KuttyJoseph A Kovacs
Nov 6, 2010·The Journal of Infectious Diseases·Geetha KuttyJoseph A Kovacs
Feb 13, 2001·Infection and Immunity·J R Stringer, S P Keely
Nov 6, 2001·American Journal of Respiratory Cell and Molecular Biology·R J Palmer, A E Wakefield
Aug 12, 2009·BMC Genomics·Scott P Keely, James R Stringer
Jun 21, 2005·Genetics·Scott P KeelyNeil Hall
Jun 10, 2010·Annual Review of Microbiology·Melanie T Cushion, James R Stringer
Feb 27, 2007·Fungal Genetics and Biology : FG & B·Scott P KeelyJames R Stringer
Feb 16, 2007·The Journal of Eukaryotic Microbiology·James R Stringer
Jan 9, 2007·Gene·Geetha Kutty, Joseph A Kovacs
Jun 14, 2015·FEMS Yeast Research·Joseph H SkalskiAndrew H Limper
Jan 4, 2012·FEMS Microbiology Reviews·Cornelis VinkH Steven Seifert
Feb 12, 2000·Fungal Genetics and Biology : FG & B·J K SchaffzinJ R Stringer
May 7, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K DalyP D Walzer
Jan 23, 2004·The Journal of Eukaryotic Microbiology·Scott P Keely, James R Stringer
Apr 25, 2006·FEMS Yeast Research·Cécile Fairhead, Bernard Dujon
Apr 21, 1999·Molecular Microbiology·E B LugliA E Wakefield
May 9, 2002·British Medical Bulletin·Ann E Wakefield
Oct 29, 1998·FEMS Immunology and Medical Microbiology·A E Wakefield
Oct 29, 1998·FEMS Immunology and Medical Microbiology·J R Stringer, M T Cushion

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.