PMID: 9537869Apr 16, 1998Paper

Resident human hepatic lymphocytes are phenotypically different from circulating lymphocytes

Journal of Hepatology
S NorrisCliona O'Farrelly

Abstract

Murine and human studies have documented the existence of subpopulations of lymphocytes in particular tissues that differ phenotypically and functionally from those in peripheral blood and may mature locally. Since little is known about lymphocyte subpopulations in the normal human liver, we have analysed the surface phenotypes of lymphocytes isolated from liver specimens taken from 15 donors at the time of liver transplantation, and compared these with those of peripheral blood lymphocytes. Hepatic lymphocytes were prepared by mechanical dissociation and enzymatic digestion of liver tissue. The cells were stained with a panel of monoclonal antibodies (CD3, CD4, CD8, CD19, CD56, gammadeltaTCR, alphabetaTCR, CD8alpha-chain, CD8alphabeta dimer), and analysed by flow cytometry. In situ characterisation of hepatic lymphocytes was by haematoxylin and eosin staining of fixed liver sections and by immunohistochemical staining for common leukocyte antigen and CD3. Significant numbers of hepatic T lymphocytes were localised to the portal tracts and parenchyma of normal liver specimens. Flow cytometry revealed that the CD4/CD8 ratio (1:3.5) was consistently reversed compared with that in peripheral blood (2:1). Other lymphocyte populatio...Continue Reading

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Citations

Mar 28, 1998·Liver Transplantation and Surgery : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·O M CrosbieJ E Hegarty
Jan 20, 2006·Journal of Cancer Research and Clinical Oncology·Xiao-Yan CaiZhao-You Tang
Nov 5, 2010·Cell and Tissue Research·Paulina M KowalewskaAlison E Fox-Robichaud
May 17, 2005·Immunology Letters·Jonathan DeanCliona O'farrelly
Nov 9, 2005·International Immunopharmacology·Yongyan ChenZhigang Tian
Feb 21, 2004·Journal of Neuroimmunology·M KakoulidouA K Lefvert
Nov 26, 2002·Journal of Hepatology·Giovanna FabioGemino Fiorelli
Mar 29, 2003·Journal of Hepatology·Stefan G Hübscher
Apr 26, 2003·Journal of Hepatology·Michelina Nascimbeni, Barbara Rehermann
Jun 23, 1999·Molecular Medicine Today·D R Jones
Jul 30, 2002·Tissue Antigens·K J L Hammond, D I Godfrey
Apr 9, 2013·Cellular & Molecular Immunology·Haoyu SunWeihua Xiao
Mar 1, 2002·Immunology and Cell Biology·Fernando A Arosa
Mar 1, 2002·Immunology and Cell Biology·Lucy Golden-Mason, Cliona O'Farrelly
May 2, 2002·Journal of Gastroenterology and Hepatology·Atsushi HokariGotaro Toda
Mar 14, 2007·The Journal of Experimental Medicine·Claire DunnMala K Maini
Nov 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Margaret James Koziel
Mar 24, 2009·Annual Review of Immunology·Ian Nicholas Crispe
Feb 13, 2004·Comparative Hepatology·Sergiusz DurowiczWaldemar L Olszewski
Nov 5, 2010·Journal of Translational Medicine·Giuseppe CastelloStefania Scala
Aug 15, 2012·Journal of Hematology & Oncology·Inga Mandac RoguljStephanie J Lee
Jan 12, 2012·PLoS Neglected Tropical Diseases·Xin HouJijia Shen
Jan 10, 2012·World Journal of Gastroenterology : WJG·Xiao-Bo ChenLu-Nan Yan
Dec 26, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Xiaoli HuJunqi Niu

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