PMID: 6970541Sep 1, 1980Paper

Residual immunogenicity of irradiated spleen cell suspensions after a single intravenous injection of sheep red blood cells in mice

Annales d'immunologie
P H LagrangeJ C Michel

Abstract

Studies were carried out to characterize the residual immunogenicity located in spleen cells of mice after one single intravenous injection of SRBC. An in vivo system was applied for initiation and expression of the immune response consisting of one intraperitoneally injection of spleen cell suspension from SRBC-injected mice in separate groups of recipients in which DTH and secondary humoral response were measured, respectively: six days or four days after the transfer of irradiated spleen cell suspension, the separate groups of recipients being either pretreated with cyclophosphamide (200 mg/kg) or primed with 5 X 10(5) SRBC. The residual immunogenicity was found to be located in a population of radio-resistant (10,000 rads) adherent spleen cells. The same population derived from naive mice injected together with the native antigen did not modify the immune response as compared with those induced by the antigen alone. Furthermore, irradiated spleen cell suspensions from SRBC-injected mice were not able to transfer adoptively DTH sensitivity in naive recipients. Accordingly to the test used to evaluate the residual immunogenicity, it was found that the kinetics of residual immunogenicity able to induce DTH was different from t...Continue Reading

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