Residual powders from Shochu distillation remnants induce apoptosis in human hepatoma cells via the caspase-independent pathway

Journal of Bioscience and Bioengineering
Masahiro OhgidaniRyuichi Ueoka

Abstract

Shochu distillation remnants (SDR) are by-products in the manufacturing process of the Japanese liquor Shochu and include various useful organic compounds derived from the fermentation of grains. We have obtained valuable powder (PSDR) from freeze-dried SDR by the treatment with ethanol. In this study, we examined the anticancer effects of barley-, rice-, and sweet potato-PSDR against HepG2 and HuH-7 cells of human hepatocellular carcinoma (HCC) in vitro. All PSDR inhibited the growth of both these HCC cells through the induction of apoptosis. Especially, barley-PSDR was the most effective for the growth inhibition and apoptosis induction of HCC cells of all PSDR. We next examined the apoptotic mechanisms induced by barley-PSDR. Decrease in mitochondrial membrane potential and release of cytochrome c from mitochondria were observed in HCC cells after the treatment with barley-PSDR. Furthermore, barley-PSDR induced the nuclear translocation of apoptosis-inducing factor (AIF) from mitochondria, while it did not significantly affect the activities of caspase-3, -8, and -9. The results suggested that barley-PSDR induced apoptosis against HCC cells via the caspase-independent mitochondrial pathway. The findings in this study suggest...Continue Reading

References

Feb 9, 2005·International Journal of Cancer. Journal International Du Cancer·Yoko MatsumotoRyuichi Ueoka
Jul 15, 2005·Nature Medicine·Guido Kroemer, Seamus J Martin
Jan 24, 2007·Physiological Reviews·Guido KroemerCatherine Brenner
Jun 16, 2007·Gastroenterology·Hashem B El-Serag, K Lenhard Rudolph
Apr 25, 2008·Journal of Agricultural and Food Chemistry·Takahiro SekiKenji Kida
Jan 27, 2010·World Journal of Gastroenterology : WJG·Piotr StefaniukAlicja Wiercinska-Drapalo
May 18, 2010·International Journal of Pharmaceutics·Hideaki IchiharaRyuichi Ueoka
Jun 25, 2010·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Marcus A Wörns, Peter R Galle
Apr 14, 2011·ACS Medicinal Chemistry Letters·Yuji KomizuRyuichi Ueoka

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