Residual red cells in blood components: A multisite study of fully automated enumeration using a hematology analyzer.

Transfusion
Chloe CavagnettoRebecca Cardigan

Abstract

Manufacture of platelet concentrates (PCs) and plasma may fail to remove all residual red blood cells (rRBCs). Measuring rRBCs for compliance to guidelines has proven challenging, leading to an absence of a consensus methodology. Sysmex hematology analyzers with the Blood Bank mode (BB mode) analysis option offer the potential for automated rRBC counting. We therefore performed a two-site appraisal of the system. Performance characteristics were determined using platelet and plasma samples spiked with RBCs. Sample stability (n = 47) and the impact of sample type were also assessed. Components (platelets, n = 1474; plasma, n = 77) prepared using different routine manufacturing methods were tested to assess variation in rRBC concentration. Linearity studies up to 19 000 RBCs/μL demonstrated good correlation between expected and observed results (R2 ≥ 0.9731), and flow cytometric results also correlated well with BB mode (R2 = 0.9400). Precision analysis gave a limit of quantitation of 6 to 7 RBCs/μL, and carryover was 0.03%. Ethylenediaminetetraacetic acid and plain tube results were not significantly different (P ≥ 0.10), and samples were stable up to 24 hours. Apheresis PCs produced at two sites had lower rRBC concentrations (m...Continue Reading

References

Oct 24, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Chérina FlemingRobert de Jonge
Mar 29, 2014·International Journal of Laboratory Hematology·C BriggsUNKNOWN International Council for Standardization of Haematology
Nov 25, 2016·Transfusion·Lucy BackholerRebecca Cardigan
Dec 4, 2016·Hematology·Jeanne E Hendrickson, Christopher A Tormey
Dec 23, 2016·British Journal of Haematology·Lise J EstcourtUNKNOWN British Committee for Standards in Haematology
Jun 22, 2018·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Johanna ReckhausBehrouz Mansouri Taleghani

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Methods Mentioned

BETA
flow cytometry
Apheresis

Software Mentioned

Sysmex
Blood Bank mode
PRISM
BB
GraphPad

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