Residue 627 of PB2 is a determinant of cold sensitivity in RNA replication of avian influenza viruses

Journal of Virology
P MassinN Naffakh

Abstract

Human influenza A viruses replicate in the upper respiratory tract at a temperature of about 33 degrees C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 degrees C. In the present study, we analyzed the influence of low temperature (33 degrees C) on RNA replication of avian and human viruses in cultured cells. The kinetics of replication of the NP segment were similar at 33 and 37 degrees C for the human A/Puerto-Rico/8/34 and A/Sydney/5/97 viruses, whereas replication was delayed at 33 degrees C compared to 37 degrees C for the avian A/FPV/Rostock/34 and A/Mallard/NY/6750/78 viruses. Making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins, we observed that the polymerase complexes derived from avian viruses but not human viruses exhibited cold sensitivity in mammalian cells, which was determined mostly by residue 627 of PB2. Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 degrees C could contribute to their inability to grow efficiently in humans.

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Citations

Mar 25, 2011·Archives of Virology·Ahmed S Abdel-MoneimAbdel-Aziz S Abu-Elsaad
Jun 6, 2013·Virus Research·Stephanie SonnbergRobert G Webster
Jun 5, 2013·Virus Research·Gülsah GabrielHans-Dieter Klenk
Sep 13, 2013·Virus Research·Bryan S Kaplan, Richard J Webby
Dec 15, 2010·Expert Reviews in Molecular Medicine·Jessica A BelserJacqueline M Katz
Nov 4, 2009·Cell Research·Gabriele NeumannYoshihiro Kawaoka
Aug 1, 2013·Emerging Microbes & Infections·Kang Yiu LaiJohn Kwok Cheung Chan
Jun 16, 2009·Nature·Gabriele NeumannYoshihiro Kawaoka
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Neal Van HoevenTerrence M Tumpey
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chengjun LiYoshihiro Kawaoka
Jun 12, 2010·The Journal of Biological Chemistry·Stéphane BoivinDarren J Hart
Dec 27, 2007·Viral Immunology·Denise A Kaminski
Apr 13, 2007·Clinical Microbiology Reviews·J S Malik PeirisYi Guan
Feb 4, 2006·Journal of Clinical Microbiology·Gabriele A LandoltChristopher W Olsen
Mar 7, 2009·Journal of Virology·Quynh Mai LeYoshihiro Kawaoka
Oct 19, 2012·Journal of Virology·Olivier MoncorgéWendy S Barclay
Mar 20, 2009·Journal of Virology·Kristy J SzretterJacqueline M Katz
Apr 24, 2009·Annual Review of Microbiology·Terrence M Tumpey, Jessica A Belser
Sep 13, 2008·Annual Review of Microbiology·Nadia NaffakhSylvie van der Werf
Jan 6, 2011·The Journal of Clinical Investigation·Donna M Tscherne, Adolfo García-Sastre
Sep 17, 2010·Virology Journal·Alongkorn AmonsinRoongroje Thanawongnuwech

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