Residue accessibility, hydrogen bonding, and molecular recognition: metal-chelate probing of active site histidines in chymotrypsins

Biochemistry
P P BernaM A Vijayalakshmi

Abstract

Subspecies defining the maturation pathway of bovine chymotrypsinogen to alpha-chymotrypsin have been separated in a single chromatographic run by affinity to iminodiacetic acid-Cu(II) [IDA-Cu(II)] immobilized onto Novarose. A major highlight of the elution pattern is that, as maturation proceeds, these subspecies exhibit a correlated increase in affinity toward IDA-Cu(II). This behavior is analyzed by a combination of physicochemical and molecular modeling techniques to assess the contribution of the two histidines present in chymotrypsins, at positions 40 and 57 on the protein surface. Catalytic His-57 features adequate surface accessibility to serve as a ligand to IDA-Cu(II), but its participation is clearly ruled out by specific chemical modification. In contrast, His-40, whose side chain is buried in the crystal structures of both zymogen and mature enzyme, surprisingly proves the most plausible candidate as an electron donor to IDA-Cu(II). This apparent conflict between histidine accessibility and their implication in IDA-Cu(II) recognition has been rationalized on the basis of their flexibility and/or hydrogen-bonding status, with the following outcome. First, histidine constitutes a useful reporter group for subtle prot...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Dec 14, 1969·Journal of Molecular Biology·M Levitt, S Lifson
Aug 5, 1994·Journal of Molecular Biology·Z S DerewendaP M Kobos
Aug 1, 1955·Biochimica Et Biophysica Acta·M ROVERYP DESNUELLE

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Citations

Mar 17, 1999·Journal of Molecular Recognition : JMR·V BodenM A Vijayalakshmi
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·G S Chaga
Aug 14, 1999·Biochimica Et Biophysica Acta·K Y JiangM A Vijayalakshmi
Jan 19, 2010·Bioinformatics·Sriram SankararamanKimmen Sjölander
Feb 3, 1999·Annals of the New York Academy of Sciences·E NedonchelleM A Vijayalakshmi
Apr 27, 2010·Journal of Chromatography. a·Rajasekar R Prasanna, Mookambeswaran A Vijayalakshmi
Dec 11, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mariana Borsoi RibeiroSonia Maria Alves Bueno
Feb 12, 2008·Biotechnology Journal·Mildred NoizetBrigitte Thomasset
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gisele SerpaSônia Maria Alves Bueno
Jul 9, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniela Todorova-BalvayMookambeswaran Vijayalakshmi
May 9, 2015·Chemical Society Reviews·Rongfeng ZouHe Tian
Jul 26, 2006·Journal of Molecular Recognition : JMR·Daniela Todorova-BalvayMookambeswaran A Vijayalakshmi
Sep 2, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elliott L RodriguezDavid S Hage
Sep 2, 2004·Journal of the American Chemical Society·Abir L BanerjeeD K Srivastava

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