PMID: 2490572Jan 1, 1989Paper

Residue determination of two co-administered antibacterial agents--cephalexin and colistin--in calf tissues using high-performance liquid chromatography and microbiological methods

Journal of Pharmaceutical and Biomedical Analysis
P LeroyA Nicolas

Abstract

Residues of two antibacterial agents, cephalexin and colistin, co-administered by intramuscular injection to calves, were quantified in four different tissues (muscle, fat, liver and kidney) by column switching HPLC and by a microbiological method. For cephalexin assay, tissue samples with cephradin as internal standard were homogenized in a 5% trichloroacetic acid solution and filtrates were injected onto a concentration precolumn filled with LiChroprep RP-18 (25-40 microns). A clean-up step was incorporated by flowing a mobile phase (methanol-0.01 M phosphate buffer (pH 3.0); 15:85, v/v) through the enrichment column before elution on a LiChrospher RP-18e (5 microns) column with a methanol-phosphate buffer (30:70, v/v) at a flow rate of 1 ml min-1. Spectrometric detection was at 260 nm. An additional "off-line" washing step of extracts with methylene chloride was operated to achieve higher selectivity in the case of liver and kidney samples. The limit for quantitative assay was 0.045 micrograms g-1 with relative standard deviations in the range 5-8% and recoveries within 70%. For microbiological assay of colistin, samples were homogenized in 0.1 M hydrochloric acid-acetonitrile mixtures (3:1, v/v, for kidney and liver; 3:2, v...Continue Reading

References

Apr 1, 1979·Journal of Pharmaceutical Sciences·T L LeeM A Brooks
Jan 11, 1978·Journal of Chromatography·T NakagawaT Uno
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·B Wiese
Jan 1, 1986·Journal of Pharmaceutical and Biomedical Analysis·C M SelavkaK Bratin

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Citations

May 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dennis J GmurPasqua A Nardella
May 26, 2006·Antimicrobial Agents and Chemotherapy·Phillip J BergenRoger L Nation
Jun 15, 2011·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Aug 12, 2009·Antimicrobial Agents and Chemotherapy·Liang JinJoseph A Nicolazzo
Feb 23, 2012·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Dec 7, 2010·Expert Opinion on Drug Metabolism & Toxicology·Argyris S MichalopoulosLeonidas Gregorakos
Mar 29, 2011·Enfermedades infecciosas y microbiología clínica·Sonia LuqueEsther Salas
Jan 12, 2011·Journal of Veterinary Pharmacology and Therapeutics·J HeX Xiao
Dec 29, 2004·International Journal of Antimicrobial Agents·Jian LiKingsley Coulthard
Jan 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Divyabharathi ChepyalaChing-Hua Kuo
Aug 26, 2014·Bioresource Technology·C RiemenschneiderG Hamscher
Aug 31, 2016·Pharmaceutical Development and Technology·Yang LiLi Yang
Jan 12, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zheng MaRobert W Milne
Oct 3, 2006·Expert Review of Anti-infective Therapy·Helen Giamarellou
Oct 4, 2012·Expert Review of Anti-infective Therapy·Silpak BiswasJean-Marc Rolain
Oct 16, 2019·Chemical Communications : Chem Comm·Ma SuJianfeng Cai
Sep 2, 2020·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Valérie GaudinChristophe Soumet
Oct 23, 2019·Journal of Animal Science and Biotechnology·Jiayi WangLidong He

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