PMID: 9435213Jan 22, 1998Paper

Residue-specific bioincorporation of non-natural, biologically active amino acids into proteins as possible drug carriers: structure and stability of the per-thiaproline mutant of annexin V

Proceedings of the National Academy of Sciences of the United States of America
N BudisaL Moroder

Abstract

Residue-specific bioincorporation of 1,3-thiazolidine-4-carboxylic acid [thiaproline, Pro(S)], a non-natural amino acid analog of proline, into human recombinant annexin V was achieved with a proline-auxotrophic Escherichia coli strain by fermentation procedures in minimal medium. Quantitative replacement of proline with thiaproline was confirmed by mass-spectrometric, amino acid, and x-ray crystallographic analyses. The wild-type protein and its per-Pro(S) mutant were found to crystallize isomorphously and to show identical three-dimensional structures in crystals. In solution the dichroic properties of the wild-type and per-Pro(S) protein confirmed nearly identical overall folds. From thermal denaturation experiments, however, a reduced Tm (-4.5 K) value was determined whereas the van't Hoff enthalpy and entropy were not significantly affected. Therefore, protein mutants containing bioactive amino acid analogs like thiaproline at multiple sites would be expected to fully retain their functional properties, including immunogenicity, and thus could serve as promising vehicles for targeted drug delivery.

References

Sep 20, 1977·Biochimica Et Biophysica Acta·C De MarcoD Cavallini
Apr 1, 1990·Trends in Biotechnology·C N Pace
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 14, 1983·Lancet·G Finsberg
May 1, 1993·Journal of Learning Disabilities·L C Moats, G R Lyon
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Apr 12, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·G LoidlL Moroder
Dec 16, 2004·Angewandte Chemie·Lei Wang, Peter G Schultz
Oct 22, 2002·Biochemical and Biophysical Research Communications·Jeffrey O BolesLouis A Silks
May 6, 2003·Journal of Molecular Biology·Jae Hyun BaeNediljko Budisa
Feb 27, 1999·Current Opinion in Biotechnology·K J Rothschild, S Gite
Nov 7, 2000·Preparative Biochemistry & Biotechnology·L N ZhangZ C Hua
Jun 23, 2001·Protein Science : a Publication of the Protein Society·N BudisaL Moroder
Jun 11, 2004·Annual Review of Biochemistry·Tamara L HendricksonPaul Schimmel
Nov 24, 2004·Biological Chemistry·Nediljko Budisa, Prajna Paramita Pal
Aug 10, 2000·Analytical Biochemistry·C MinksN Budisa
May 1, 2012·Biochemical and Biophysical Research Communications·Maud LarregolaNediljko Budisa
Feb 17, 2009·Biophysical Journal·Aaron P YamniukHans J Vogel
Aug 27, 2014·The Journal of Physical Chemistry. B·Yu-Ju LinJia-Cherng Horng
May 21, 2004·Protein Science : a Publication of the Protein Society·Vincenzo De FilippisAngelo Fontana
Jul 9, 2004·Chembiochem : a European Journal of Chemical Biology·Wookhyun KimVincent P Conticello
Jun 8, 2006·Chembiochem : a European Journal of Chemical Biology·Soojin SonDavid A Tirrell
Jun 9, 2006·The FEBS Journal·Malgorzata Giel-Pietraszuk, Jan Barciszewski
Mar 11, 2008·Organic & Biomolecular Chemistry·Bagher Amir-HeidariJason Micklefield
Jul 16, 2010·Macromolecular Bioscience·Xin HuangJunqiu Liu
Mar 2, 2001·Angewandte Chemie·Christian RennerLuis Moroder
Mar 21, 2006·Biotechnology and Bioengineering·Tatyana PanchenkoJin Kim Montclare
Dec 20, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Luis Moroder, Hans-Jürgen Musiol
Jul 10, 2002·The Journal of Biological Chemistry·Christopher S HayesRobert T Sauer
Sep 30, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·George L OrrissJörg Stetefeld
Jun 15, 2011·Chembiochem : a European Journal of Chemical Biology·Diya M AbdeljabbarA James Link
Apr 18, 2020·The Biochemical Journal·Jenelle A PattersonAndrew D Hanson
Dec 13, 2017·Biotechnology and Applied Biochemistry·Vincenzo De FilippisDaniele Peterle
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N BudisaR Huber
Dec 22, 2009·Molecular BioSystems·Natalya Voloshchuk, Jin Kim Montclare
Sep 17, 2020·Natural Product Reports·Manuel Montalbán-LópezWilfred A van der Donk
Aug 21, 2021·Chembiochem : a European Journal of Chemical Biology·Matthew C T Hartman
Jul 10, 2003·Chemical Reviews·Christophe Dugave, Luc Demange

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