PMID: 8597567Feb 8, 1996Paper

Residues in Torpedo californica acetylcholinesterase necessary for processing to a glycosyl phosphatidylinositol-anchored form

Biochimica Et Biophysica Acta
G Bucht, K Hjalmarsson

Abstract

Acetylcholinesterase from Torpedo californica (TcAChE) can be found as a glycosyl phosphatidylinositol (GPI)-anchored, membrane associated form. The C-terminal amino-acid sequence of the precursor protein resembles the signal peptide sequence found in proteins and enzymes destined for GPI-modification. Characteristics of such a signal peptide are a relatively polar stretch of amino acids, separating a cleavage- and modification-site (omega-site) residue from a hydrophobic C-terminus. We have introduced mutations, both at putative omega-sites and in the hydrophobic region, and analysed their effects on GPI-anchoring of TcAChE. Our results show that substitution of all three Ser residues in the region Ser542-Ser544 prevents GPI-modification and membrane anchoring. Individual substitution of each of these residues resulted in no or only a minor effect on the modification. We therefore conclude that more than one residue within this sequence can be utilised as the omega-site. Our analyses of double substitutions indicated that Ser543 and Ser544 are the preferred residues for GPI-modification. Moreover, the hydrophobic region is shown to be essential for GPI-anchoring of TcAChE.

References

Jun 12, 1990·Biochemistry·J R ThomasT W Rademacher
Apr 1, 1989·The Journal of Cell Biology·I W CarasS R Williams
Feb 7, 1985·Nature·T SekiJ Silver
Feb 1, 1988·The Journal of Experimental Medicine·L L Button, W R McMaster
Jan 30, 1987·Biochemical and Biophysical Research Communications·S OikawaG Kosaki
Jun 5, 1983·Journal of Molecular Biology·P A MichelsJ H Van Boom
Aug 30, 1994·Biochemical and Biophysical Research Communications·H ChaabihiM Cérutti
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

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Citations

Jan 10, 2003·Transfusion·Marion E Reid
Feb 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew K JonesAlison Agnew
Aug 10, 2005·Transfusion·Marion E Reid
Sep 25, 1999·Journal of Molecular Biology·B EisenhaberF Eisenhaber
Oct 28, 1998·Journal of Physiology, Paris·J MassouliéS Simon
May 14, 2003·Molecular and Biochemical Parasitology·Jun WangGeorge A M Cross

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