Abstract
Interaction between the gamma subunit (Pgamma) of cGMP phosphodiesterase and the alpha subunit (Talpha) of transducin is a key step for the regulation of cGMP phosphodiesterase in retinal rod outer segments. Here we have utilized a combination of specific modification by an endogenous enzyme and site-directed mutagenesis of the Pgamma polycationic region to identify residues required for the interaction with Talpha. Pgamma, free or complexed with the alphabeta subunit (Palphabeta) of cGMP phosphodiesterase, was specifically radiolabeled by prewashed rod membranes in the presence of [adenylate-32P]NAD. Identification of ADP-ribose in the radiolabeled Pgamma and radiolabeling of arginine-replaced mutant forms of Pgamma indicate that both arginine 33 and arginine 36 are similarly ADP-ribosylated by endogenous ADP-ribosyltransferase, but only one arginine is modified at a time. Pgamma complexed with Talpha (both GTP- and GDP-bound forms) was not ADP-ribosylated; however, agmatine, which cannot interact with Talpha, was ADP-ribosylated in the presence of Talpha, suggesting that a Pgamma domain containing these arginines is masked by Talpha. A Pgamma mutant (R33,36K), as well as wild type Pgamma, inhibited both GTP hydrolysis of Talp...Continue Reading
References
Jun 30, 1992·Biochemistry·R L Brown
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A ZolkiewskaJ Moss
Jan 1, 1992·International Review of Cytology·P A Hargrave, J H McDowell
Sep 14, 1992·FEBS Letters·S Ehret-HilbererN Virmaux
Aug 15, 1991·European Journal of Biochemistry·S ObaraM Shimoyama
Jan 1, 1991·Methods in Enzymology·J E Casnellie
Jul 18, 1988·FEBS Letters·V M LipkinR N Etingof
Jan 1, 1989·Annual Review of Neuroscience·K W Yau, D A Baylor
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P G Gillespie, J A Beavo
Oct 5, 1987·FEBS Letters·D F MorrisonD J Takemoto
Apr 29, 1987·Biochemical and Biophysical Research Communications·N HaraM Shimoyama
Aug 18, 1986·FEBS Letters· Ovchinnikov YuAK G Muradov
Jan 1, 1987·Annual Review of Physiology·J B Hurley
Jan 1, 1986·Annual Review of Cell Biology·L Stryer, H R Bourne
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A YamazakiM W Bitensky
Jun 2, 1995·The Journal of Biological Chemistry·N P SkibaH E Hamm
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·R H CoteV Y Arshavsky
Mar 1, 1993·Biochimica Et Biophysica Acta·E N Pugh, T D Lamb
Apr 16, 1993·Biochimica Et Biophysica Acta·V M Lipkin Natochin MYu
Aug 24, 1993·Biochemistry·A Otto-BrucM Chabre
Apr 30, 1993·Biochemical and Biophysical Research Communications·N PozdnyakovA Sitaramayya
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·H HtunG L Hager
May 15, 1996·Biochemical and Biophysical Research Communications·A Boldyrev, E Kurella
May 15, 1996·Biochemical and Biophysical Research Communications·A YamazakiH Matsumoto
Dec 20, 1996·The Journal of Biological Chemistry·A YamazakiF Hayashi
Citations
Jan 30, 2002·The Journal of General Physiology·Peter D CalvertClint L Makino
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Paul O HassaMichael O Hottiger
Jun 6, 2014·Nature Reviews. Cancer·Sejal Vyas, Paul Chang
Sep 24, 2004·Neurochemistry International·John W Wellard, Ian G Morgan
Mar 25, 2017·International Journal of Molecular Sciences·Eric P KnottDamien D Pearse
Dec 14, 2001·The Journal of Biological Chemistry·Michael J PagliaRick H Cote
Jul 8, 2000·The Journal of Biological Chemistry·I MatsuuraA Yamazaki
Jul 8, 2000·The Journal of Biological Chemistry·F HayashiA Yamazaki
Aug 15, 2002·The Journal of Biological Chemistry·Matsuyo YamazakiAkio Yamazaki
Mar 29, 2011·The FEBS Journal·Akio YamazakiVladimir A Bondarenko
Feb 13, 2010·Molecular and Cellular Biochemistry·Akio YamazakiJiro Usukura
May 26, 2001·The Journal of Biological Chemistry·H Mou, R H Cote
Feb 24, 2010·Molecular and Cellular Biochemistry·Akio YamazakiJiro Usukura
Jul 1, 1999·Biochemistry·V A BondarenkoA Yamazaki
May 23, 1998·Biochemistry·L X XuF Hayashi