PMID: 6170617Jan 1, 1981Paper

Resin embedment of organs and postembedment localization of antigens by immunoperoxidase methods

Histochemistry
W D Kuhlmann, R Krischan

Abstract

Various procedures for nonpolar and polar resin embedment were applied to mouse and rat livers for the study of postembedment immunolocalization of alpha 1-fetoprotein, albumin and the microsomal enzyme epoxide hydrolase. Fixations with formaldehyde and with formaldehyde-glutaraldehyde mixtures were used for tissue stabilization. Both fixation schedules did not abolish immunoreactivity. Treatment of liver with inert compounds such as polyvinylpyrrolidones or chemical modification of antigens with ethyl acetimidate prior to embedment improved immuno-staining. Either the low-polarity solvent ethanol or the highly polar ethylene glycol could be employed as dehydrating agents. Antigens were readily localized in sections from Epon 812 embedded livers. For this purpose, polymerized resin had to be partially removed. On the other hand, immunoreactivity of antigens was only faint after embedment in an epoxy-resin based on diepoxide octane. Also, antigens reacted faintly in sections from livers which were embedded at 0 degrees C in the polar acrylate-methacrylate based Lowicryl K4M resin. The indirect peroxidase labelled antibody method was as specific and sensitive as the PAP technique. Optimal antigen detection was attained with antib...Continue Reading

References

Jan 1, 1977·Progress in Histochemistry and Cytochemistry·W D Kuhlmann
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Sep 15, 1978·International Journal of Cancer. Journal International Du Cancer·W D Kuhlmann
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Citations

Mar 30, 1984·Journal of Immunological Methods·R M Franklin
May 1, 1984·Collagen and Related Research·W A Horton
Jan 1, 1989·Anatomy and Embryology·C M Griffith, M J Wiley
Jan 1, 1985·Progress in Histochemistry and Cytochemistry·J A Litwin
Jan 1, 1995·Progress in Histochemistry and Cytochemistry·M Bendayan
Sep 13, 2006·International Journal of Experimental Pathology·W D Kuhlmann, Peter Peschke
Jan 1, 1983·Annals of the New York Academy of Sciences·R M Franklin

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