Resistance factor-mediated streptomycin resistance.

Journal of Bacteriology
J H Harwood, D H Smith

Abstract

Resistance (R) factor-mediated streptomycin (Sm) resistance differs from classical, high-level, chromosome-borne Sm resistance in its dominance over sensitivity and in the level of its effectiveness (in Escherichia coli approximately 25 mug/ml versus >2,000 mug/ml). In addition, an R factor-containing strain, unlike high-level Sm-resistant bacteria, showed an inoculum effect with respect to its level of Sm resistance. Crude extracts of this strain destroyed the inhibitory activity of Sm and bluensomycin (Blue) on in vitro protein synthesis. The ribosomes from this strain proved to be sensitive to Sm in vitro. The requirements for in vitro inactivation of Sm (and Blue) were determined to be: extract, adenosine triphosphate or deoxyadenosinetriphosphate, and Mg(++). Chromatographic techniques with radioisotopes revealed the formation of an inactivated form of Sm containing adenosine (or deoxyadenosine), phosphate, and Sm in equimolar amounts. The adenylate moiety is coupled to the streptobiosamine residue, rather than to the streptidine ring, of the Sm molecule. The adenylating enzyme, which is not induced by Sm, is located in the periplasmic space of the R factor-containing strain.

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Citations

Dec 1, 1969·Genetical Research·J H Harwood, D H Smith
Jun 11, 1971·Annals of the New York Academy of Sciences·T Watanabe
Jan 1, 1972·Japanese Journal of Microbiology·F KobayashiS Mitsuhashi
Nov 1, 1971·Japanese Journal of Microbiology·H Kawabe, S Mitsuhashi
Dec 1, 1970·Journal of Bacteriology·K Carlson, R C Bockrath
Feb 28, 2002·Medical Hypotheses·J D Kornder
Jul 1, 1974·Journal of Bacteriology·A O Summers, L I Sugarman
Jan 1, 1970·Infection and Immunity·D H SmithP W Anderson
Jun 1, 1974·Antimicrobial Agents and Chemotherapy·H KawabeS Mitsuhashi
Jan 1, 1982·Journal of Bacteriology·A O SummersC Slater
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Jul 1, 1971·Mutation Research·D Ewing
Nov 1, 1969·Journal of Bacteriology·P Anderson
Jan 1, 1972·Antimicrobial Agents and Chemotherapy·L E BryanJ T Tseng
Feb 1, 1972·Antimicrobial Agents and Chemotherapy·M YamaguchiS Mitsuhashi
Oct 11, 1969·Lancet·P GardnerR C Moellering
Jan 8, 1971·Biochemical and Biophysical Research Communications·J Harwood, D H Smith
May 19, 1972·Science·J E Davies, R Rownd

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