Resistance in marine cyanobacteria differs against specialist and generalist cyanophages

Proceedings of the National Academy of Sciences of the United States of America
Sophia Zborowsky, Debbie Lindell

Abstract

Long-term coexistence between unicellular cyanobacteria and their lytic viruses (cyanophages) in the oceans is thought to be due to the presence of sensitive cells in which cyanophages reproduce, ultimately killing the cell, while other cyanobacteria survive due to resistance to infection. Here, we investigated resistance in marine cyanobacteria from the genera Synechococcus and Prochlorococcus and compared modes of resistance against specialist and generalist cyanophages belonging to the T7-like and T4-like cyanophage families. Resistance was extracellular in most interactions against specialist cyanophages irrespective of the phage family, preventing entry into the cell. In contrast, resistance was intracellular in practically all interactions against generalist T4-like cyanophages. The stage of intracellular arrest was interaction-specific, halting at various stages of the infection cycle. Incomplete infection cycles proceeded to various degrees of phage genome transcription and translation as well as phage genome replication in numerous interactions. In a particularly intriguing case, intracellular capsid assembly was observed, but the phage genome was not packaged. The cyanobacteria survived the encounter despite late-stag...Continue Reading

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Citations

Sep 17, 2020·Environmental Microbiology·Matthew J Tuttle, Alison Buchan
Jan 17, 2020·Scientific Reports·Tzvi HoltzmanUdi Qimron
Mar 26, 2021·Nature Reviews. Microbiology·Adrienne M S CorreaJoshua S Weitz

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Datasets Mentioned

BETA
MIT9215
MITS9902
PXD013030

Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
reverse-phase chromatography
ICE
PCR

Software Mentioned

LightCycler 480
Proteome Discoverer
MaxQuant
ImageJ
SPSS

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