PMID: 8218Aug 1, 1976

Resistance of alkylated DNA to degradation by deoxyribonuclease II at neutral and acid pH

Chemico-biological Interactions
P J O'ConnorG P Margison

Abstract

Methylation of a calf thymus DNA substrate by dimethyl sulphate (DMS) leads to an inhibition of deoxyribonuclease II activity which is gradually lost with time. The extent of this initial inhibition is linearly related to the amount of methylated products in DNA and quantitatively similar effects were found when the enzyme was used under either acid or neutral conditions. Deoxyribonuclease II was shown to produce 3'-phosphate termini under both acid and neutral conditions and thus, irrespective of the ionic conditions for the action of this enzyme in vivo the effects demonstrated here are of potential significance. Local denaturation of the methylated DNA may be partly responsible for these inhibitory effects but it is likely that the methyl purines also play a more direct role.

References

Dec 21, 1973·Biochimica Et Biophysica Acta·G P Margison, P J O'Connor
Nov 21, 1972·European Journal of Biochemistry·M E HainesI R Johnston
Jul 1, 1972·The Biochemical Journal·G P Margison, P J O'Connor
Oct 18, 1973·European Journal of Biochemistry·A Devillers-ThieryG Bernardi
Oct 14, 1968·Journal of Molecular Biology·G Bernardi, M L Bach
Jul 15, 1971·European Journal of Biochemistry·J RamsteinM Leng
Aug 1, 1971·The Biochemical Journal·H Slor, T Lev
Nov 21, 1967·Biochimica Et Biophysica Acta·F Pochon, A M Michelson
Jul 1, 1961·Archives of Biochemistry and Biophysics·W OSTROWSKI, A TSUGITA
Jul 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·E C RAUENBUSCH, K I ALTMAN

Related Concepts

Alkylation
Bos indicus
Alkaline DNase
DNA, Double-Stranded
DNA Nucleotidyltransferases
Hydrogen-Ion Concentration
RNA Denaturation
Pancreas
Spleen
Genetic Template

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