PMID: 6406685Jun 1, 1983Paper

Resistance of bacteriophage H1 to restriction and modification by Bacillus subtilis R

Journal of Virology
S BronG Venema

Abstract

H1, a 5-hydroxymethyluracil (HMU)-containing Bacillus subtilis bacteriophage, was neither restricted nor modified upon infection of B. subtilis R cells. In vitro, H1 DNA was not restricted by BsuR under standard conditions (200 mM salt), although the expected frequency of -GGCC- cleavage sites was approximately 250. However, four specific sites were cleaved under nonstandard conditions (low salt or high pH) or in the presence of organic solvents, like dimethyl sulfoxide and glycerol. After the substitution of thymine for HMU by DNA cloning in B. subtilis, a BsuR cleavage site was restricted and modified under standard conditions. No additional sites were detected after shotgun-cloning of about 11% of the chromosome. The nucleotide sequence of a cleavage site was found to be 5'. .C-A-Hmu-A-A-C-Hmu-Hmu-Hmu-G-G-C-C-Hmu-A-G-. . .3', which shows the presence of a bona fide BsuR (GGCC) recognition sequence, flanked by (Hmu-A)-rich sequences. The results suggested that the resistance of H1 to restriction and modification by B. subtilis R was due to (i) a strong bias against the GGCC-recognition sequence and (ii) protection of the four remaining GGCC sites as a consequence of HMU-A base pairs flanking the sites.

References

Jan 1, 1979·Gene·J Ito, R J Roberts
Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Mar 1, 1979·Journal of Virology·E F WagnerM Schweiger
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S D Ehrlich
Nov 1, 1976·CRC Critical Reviews in Biochemistry·R J Roberts
Jan 1, 1974·Molecular & General Genetics : MGG·T A TrautnerC Anagnostopoulos
Jan 1, 1974·Progress in Nucleic Acid Research and Molecular Biology·W Arber
Jan 1, 1971·Annual Review of Microbiology·H W Boyer
Jan 1, 1980·Methods in Enzymology·S Bron, W Hörz
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Mar 1, 1981·Microbiological Reviews·D H Krüger, C Schroeder
Feb 11, 1982·Nucleic Acids Research·J E Brooks, R J Roberts
Mar 11, 1982·Nucleic Acids Research·L H HuangM Ehrlich
Jan 1, 1980·Annual Review of Microbiology·R A Warren

❮ Previous
Next ❯

Citations

Jun 26, 2009·Chemistry & Biodiversity·Piet Herdewijn, Philippe Marlière
Oct 16, 1999·Nucleic Acids Research·H M VuD R Kearns
Mar 1, 1990·Archives of Environmental Health·J J SteinbergD Gil
Sep 25, 2017·Angewandte Chemie·Thomas CarellFabio Spada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.